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1sf2

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{{STRUCTURE_1sf2| PDB=1sf2 | SCENE= }}
{{STRUCTURE_1sf2| PDB=1sf2 | SCENE= }}
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'''Structure of E. coli gamma-aminobutyrate aminotransferase'''
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===Structure of E. coli gamma-aminobutyrate aminotransferase===
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==Overview==
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The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A resolution for the unliganded enzyme and 1.9 A resolution for the aminooxyacetate complex. The overall structure of the enzyme is similar to those of other aminotransferase subgroup II enzymes. The ability of gamma-aminobutyrate aminotransferase to act on primary amine substrates (gamma-aminobutyrate) in the first half-reaction and alpha-amino acids in the second is proposed to be enabled by the presence of Glu211, whose side chain carboxylate alternates between interactions with Arg398 in the primary amine half-reaction and an alternative binding site in the alpha-amino acid half-reaction, in which Arg398 binds the substrate alpha-carboxylate. The specificity for a carboxylate group on the substrate side chain is due primarily to the presence of Arg141, but also requires substantial local main chain rearrangements relative to the structurally homologous enzyme dialkylglycine decarboxylase, which is specific for small alkyl side chains. No iron-sulfur cluster is found in the bacterial enzyme as was found in the pig enzyme [Storici, P., De Biase, D., Bossa, F., Bruno, S., Mozzarelli, A., Peneff, C., Silverman, R. B., and Schirmer, T. (2004) J. Biol. Chem. 279, 363-73.]. The binding of aminooxyacetate causes remarkably small changes in the active site structure, and no large domain movements are observed. Active site structure comparisons with pig gamma-aminobutyrate aminotransferase and dialkylglycine decarboxylase are discussed.
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{{ABSTRACT_PUBMED_15323550}}
==About this Structure==
==About this Structure==
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[[Category: Zhou, X.]]
[[Category: Zhou, X.]]
[[Category: Aminotransferase]]
[[Category: Aminotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:37:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:13:45 2008''

Revision as of 10:13, 29 July 2008

Image:1sf2.png

Template:STRUCTURE 1sf2

Structure of E. coli gamma-aminobutyrate aminotransferase

Template:ABSTRACT PUBMED 15323550

About this Structure

1SF2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase., Liu W, Peterson PE, Carter RJ, Zhou X, Langston JA, Fisher AJ, Toney MD, Biochemistry. 2004 Aug 31;43(34):10896-905. PMID:15323550

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