9bw1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==TnsABCD-DNA transpososome== | |
| + | <StructureSection load='9bw1' size='340' side='right'caption='[[9bw1]], [[Resolution|resolution]] 3.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9bw1]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Peltigera_membranacea Peltigera membranacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BW1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.65Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bw1 OCA], [https://pdbe.org/9bw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bw1 RCSB], [https://www.ebi.ac.uk/pdbsum/9bw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bw1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A235IGG7_9NOSO A0A235IGG7_9NOSO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Tn7-like transposons are characterized by their ability to insert specifically into host chromosomes. Recognition of the attachment (att) site by TnsD recruits the TnsABC proteins to form the transpososome and facilitate transposition. Although this pathway is well established, atomic-level structural insights of this process remain largely elusive. Here, we present the cryo-electron microscopy (cryo-EM) structures of the TnsC-TnsD-att DNA complex and the TnsABCD transpososome from the Tn7-like transposon in Peltigera membranacea cyanobiont 210A, a type I-B CRISPR-associated transposon. Our structures reveal a striking bending of the att DNA, featured by the intercalation of an arginine side chain of TnsD into a CC/GG dinucleotide step. The TnsABCD transpososome structure reveals TnsA-TnsB interactions and demonstrates that TnsC not only recruits TnsAB but also directly participates in the transpososome assembly. These findings provide mechanistic insights into targeted DNA insertion by Tn7-like transposons, with implications for improving the precision and efficiency of their genome-editing applications. | ||
| - | + | Structure of TnsABCD transpososome reveals mechanisms of targeted DNA transposition.,Wang S, Siddique R, Hall MC, Rice PA, Chang L Cell. 2024 Oct 8:S0092-8674(24)01071-7. doi: 10.1016/j.cell.2024.09.023. PMID:39383864<ref>PMID:39383864</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9bw1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Peltigera membranacea]] | ||
| + | [[Category: Chang L]] | ||
| + | [[Category: Wang S]] | ||
Current revision
TnsABCD-DNA transpososome
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