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1sgk

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{{STRUCTURE_1sgk| PDB=1sgk | SCENE= }}
{{STRUCTURE_1sgk| PDB=1sgk | SCENE= }}
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'''NUCLEOTIDE-FREE DIPHTHERIA TOXIN'''
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===NUCLEOTIDE-FREE DIPHTHERIA TOXIN===
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==Overview==
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The crystal structure of diphtheria toxin (DT) in the absence of nucleotide (nucleotide-free DT) has been determined at 2.3 A resolution to a crystallographic R factor and free R factor of 18.2 and 28.2%, respectively. A comparison of this structure to the previously determined structures of DT in complex with adenyly(3'-5')uridine monophosphate (ApUp) and DT in complex with nicotinamide adenine dinucleotide (NAD) reveals that there are no significant movements of the two subdomains of the catalytic (C) domain associated with dinucleotide binding. The side chains of six residues within the active-site cleft, including Tyr65, Pro38, Tyr27, Thr23, Glu148, and Tyr54, show movements of up to 3 A upon dinucleotide binding. In the structure of nucleotide-free DT, the active-site loop residues 39-47 of the C domain are well ordered and extend over the active-site cleft in approximately the same position as in the structure of DT in complex with ApUp. This is in contrast to the structure of the DT-NAD complex, in which the active-site loop is disordered. On the basis of a comparison of the nucleotide-free and NAD-bound DT structures, we suggest that the interaction of NAD with Pro38 and also possibly Tyr54 and Trp153 could disrupt the network of hydrogen bonds that stabilizes the position of the active-site loop over the active-site cleft, allowing this loop to become disordered. This may be an important step in binding of the C domain of DT to its substrate, elongation factor-2.
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(as it appears on PubMed at http://www.pubmed.gov), where 9012663 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9012663}}
==About this Structure==
==About this Structure==
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[[Category: Toxin]]
[[Category: Toxin]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:40:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:34:15 2008''

Revision as of 12:34, 27 July 2008

Image:1sgk.png

Template:STRUCTURE 1sgk

NUCLEOTIDE-FREE DIPHTHERIA TOXIN

Template:ABSTRACT PUBMED 9012663

About this Structure

1SGK is a Single protein structure of sequence from Corynephage beta. Full crystallographic information is available from OCA.

Reference

Crystal structure of nucleotide-free diphtheria toxin., Bell CE, Eisenberg D, Biochemistry. 1997 Jan 21;36(3):481-8. PMID:9012663

Page seeded by OCA on Sun Jul 27 15:34:15 2008

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