1siw

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[[Image:1siw.gif|left|200px]]
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{{STRUCTURE_1siw| PDB=1siw | SCENE= }}
{{STRUCTURE_1siw| PDB=1siw | SCENE= }}
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'''Crystal structure of the apomolybdo-NarGHI'''
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===Crystal structure of the apomolybdo-NarGHI===
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==Overview==
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We have used EPR spectroscopy, redox potentiometry, and protein crystallography to characterize the [4Fe-4S] cluster (FS0) of the Escherichia coli nitrate reductase A (NarGHI) catalytic subunit (NarG). FS0 is clearly visible in the crystal structure of NarGHI [Bertero, M. G., et al. (2003) Nat. Struct. Biol. 10, 681-687] but has novel coordination comprising one His residue and three Cys residues. At low temperatures (&lt;15 K), reduced NarGHI exhibits a previously unobserved EPR signal comprising peaks at g = 5.023 and g = 5.556. We have assigned these features to a [4Fe-4S](+) cluster with an S = (3)/(2) ground state, with the g = 5.023 and g = 5.556 peaks corresponding to subpopulations exhibiting DeltaS = (1)/(2) and DeltaS = (3)/(2) transitions, respectively. Both peaks exhibit midpoint potentials of approximately -55 mV at pH 8.0 and are eliminated in the EPR spectrum of apomolybdo-NarGHI. The structure of apomolybdo-NarGHI reveals that FS0 is still present but that there is significant conformational disorder in a segment of residues that includes one of the Cys ligands. On the basis of these observations, we have assigned the high-spin EPR features of reduced NarGHI to FS0.
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{{ABSTRACT_PUBMED_15122898}}
==About this Structure==
==About this Structure==
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[[Category: Electron-transfer]]
[[Category: Electron-transfer]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:45:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:07:35 2008''

Revision as of 09:07, 29 July 2008

Image:1siw.png

Template:STRUCTURE 1siw

Crystal structure of the apomolybdo-NarGHI

Template:ABSTRACT PUBMED 15122898

About this Structure

1SIW is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The catalytic subunit of Escherichia coli nitrate reductase A contains a novel [4Fe-4S] cluster with a high-spin ground state., Rothery RA, Bertero MG, Cammack R, Palak M, Blasco F, Strynadka NC, Weiner JH, Biochemistry. 2004 May 11;43(18):5324-33. PMID:15122898

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