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| - | [[Image:1sj2.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1sj2| PDB=1sj2 | SCENE= }} | | {{STRUCTURE_1sj2| PDB=1sj2 | SCENE= }} |
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| - | '''Crystal structure of Mycobacterium tuberculosis catalase-peroxidase'''
| + | ===Crystal structure of Mycobacterium tuberculosis catalase-peroxidase=== |
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| - | ==Overview==
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| - | The Mycobacterium tuberculosis catalase-peroxidase is a multifunctional heme-dependent enzyme that activates the core anti-tuberculosis drug isoniazid. Numerous studies have been undertaken to elucidate the enzyme-dependent mechanism of isoniazid activation, and it is well documented that mutations that reduce activity or inactivate the catalase-peroxidase lead to increased levels of isoniazid resistance in M. tuberculosis. Interpretation of the catalytic activities and the effects of mutations upon the action of the enzyme to date have been limited due to the lack of a three-dimensional structure for this enzyme. In order to provide a more accurate model of the three-dimensional structure of the M. tuberculosis catalase-peroxidase, we have crystallized the enzyme and now report its crystal structure refined to 2.4-A resolution. The structure reveals new information about dimer assembly and provides information about the location of residues that may play a role in catalysis including candidates for protein-based radical formation. Modeling and computational studies suggest that the binding site for isoniazid is located near the delta-meso heme edge rather than in a surface loop structure as currently proposed. The availability of a crystal structure for the M. tuberculosis catalase-peroxidase also permits structural and functional effects of mutations implicated in causing elevated levels of isoniazid resistance in clinical isolates to be interpreted with improved confidence. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15231843}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15231843 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15231843}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Raven, E L.]] | | [[Category: Raven, E L.]] |
| | [[Category: Homodimer]] | | [[Category: Homodimer]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:45:48 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:38:37 2008'' |
Revision as of 01:38, 29 July 2008
Template:STRUCTURE 1sj2
Crystal structure of Mycobacterium tuberculosis catalase-peroxidase
Template:ABSTRACT PUBMED 15231843
About this Structure
1SJ2 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Crystal structure of Mycobacterium tuberculosis catalase-peroxidase., Bertrand T, Eady NA, Jones JN, Jesmin, Nagy JM, Jamart-Gregoire B, Raven EL, Brown KA, J Biol Chem. 2004 Sep 10;279(37):38991-9. Epub 2004 Jul 1. PMID:15231843
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