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1sj4

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{{STRUCTURE_1sj4| PDB=1sj4 | SCENE= }}
{{STRUCTURE_1sj4| PDB=1sj4 | SCENE= }}
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'''Crystal structure of a C75U mutant Hepatitis Delta Virus ribozyme precursor, in Cu2+ solution'''
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===Crystal structure of a C75U mutant Hepatitis Delta Virus ribozyme precursor, in Cu2+ solution===
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==Overview==
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Ribozymes enhance chemical reaction rates using many of the same catalytic strategies as protein enzymes. In the hepatitis delta virus (HDV) ribozyme, site-specific self-cleavage of the viral RNA phosphodiester backbone requires both divalent cations and a cytidine nucleotide. General acid-base catalysis, substrate destabilization and global and local conformational changes have all been proposed to contribute to the ribozyme catalytic mechanism. Here we report ten crystal structures of the HDV ribozyme in its pre-cleaved state, showing that cytidine is positioned to activate the 2'-OH nucleophile in the precursor structure. This observation supports its proposed role as a general base in the reaction mechanism. Comparison of crystal structures of the ribozyme in the pre- and post-cleavage states reveals a significant conformational change in the RNA after cleavage and that a catalytically critical divalent metal ion from the active site is ejected. The HDV ribozyme has remarkable chemical similarity to protein ribonucleases and to zymogens for which conformational dynamics are integral to biological activity. This finding implies that RNA structural rearrangements control the reactivity of ribozymes and ribonucleoprotein enzymes.
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The line below this paragraph, {{ABSTRACT_PUBMED_15141216}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15141216 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15141216}}
==About this Structure==
==About this Structure==
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[[Category: Rna]]
[[Category: Rna]]
[[Category: U1a]]
[[Category: U1a]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:45:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:04:57 2008''

Revision as of 13:04, 27 July 2008

Image:1sj4.png

Template:STRUCTURE 1sj4

Crystal structure of a C75U mutant Hepatitis Delta Virus ribozyme precursor, in Cu2+ solution

Template:ABSTRACT PUBMED 15141216

About this Structure

1SJ4 is a Single protein structure of sequence from Hepatitis delta virus and Homo sapiens. Full crystallographic information is available from OCA.

Reference

A conformational switch controls hepatitis delta virus ribozyme catalysis., Ke A, Zhou K, Ding F, Cate JH, Doudna JA, Nature. 2004 May 13;429(6988):201-5. PMID:15141216

Page seeded by OCA on Sun Jul 27 16:04:57 2008

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