1sjv

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[[Image:1sjv.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1sjv| PDB=1sjv | SCENE= }}
{{STRUCTURE_1sjv| PDB=1sjv | SCENE= }}
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'''Three-Dimensional Structure of a Llama VHH Domain Swapping'''
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===Three-Dimensional Structure of a Llama VHH Domain Swapping===
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==Overview==
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Among mammals, camelids have a unique immunological system since they produce functional antibodies devoid of light chains and CH1 domains. To bind antigens, whether they are proteins or haptens, camelids use the single domain VH from their heavy chain (VHH). We report here on such a llama VHH domain (VHH-R9) which was raised against a hapten, the RR6 red dye. This VHH possesses the shortest complementarity determining region 3 (CDR3) among all the known VHH sequences and nevertheless binds RR6 efficiently with a K(d) value of 83 nM. However, the crystal structure of VHH-R9 exhibits a striking feature: its CDR3 and its last beta-strand (beta9) do not follow the immunoglobulin VH domain fold, but instead extend out of the VHH molecular boundary and associate with a symmetry-related molecule. The two monomers thus form a domain-swapped dimer which establishes further contacts with symmetry-related molecules and build a crystal-wide beta-sheet structure. The driving force of the dimer formation is probably the strain induced by the short CDR3 together with the cleavage of the first seven residues.
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The line below this paragraph, {{ABSTRACT_PUBMED_15094039}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15094039 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15094039}}
==About this Structure==
==About this Structure==
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[[Category: Domain swapping]]
[[Category: Domain swapping]]
[[Category: Heavy chain antibody]]
[[Category: Heavy chain antibody]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:47:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:29:04 2008''

Revision as of 05:29, 28 July 2008

Image:1sjv.png

Template:STRUCTURE 1sjv

Three-Dimensional Structure of a Llama VHH Domain Swapping

Template:ABSTRACT PUBMED 15094039

About this Structure

1SJV is a Single protein structure of sequence from Lama glama. Full crystallographic information is available from OCA.

Reference

Domain swapping of a llama VHH domain builds a crystal-wide beta-sheet structure., Spinelli S, Desmyter A, Frenken L, Verrips T, Tegoni M, Cambillau C, FEBS Lett. 2004 Apr 23;564(1-2):35-40. PMID:15094039

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