1sjx

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{{STRUCTURE_1sjx| PDB=1sjx | SCENE= }}
{{STRUCTURE_1sjx| PDB=1sjx | SCENE= }}
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'''Three-Dimensional Structure of a Llama VHH Domain OE7 binding the cell wall protein Malf1'''
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===Three-Dimensional Structure of a Llama VHH Domain OE7 binding the cell wall protein Malf1===
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==Overview==
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As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein of Malassezia furfur in the presence of shampoo. We found that phage display of llama single-domain antibody fragments (VHHs) can be extended to very harsh conditions, such as the presence of shampoo containing nonionic and anionic surfactants. We selected several VHHs that bind to the cell wall protein Malf1 of M. furfur, a fungus implicated in causing dandruff. In addition to high stability in the presence of shampoo, these VHHs are also stable under other denaturing conditions, such as high urea concentrations. Many of the stable VHHs were found to contain arginine at position 44. Replacement of the native amino acid at position 44 with arginine in the most stable VHH that lacked this arginine resulted in a dramatic further increase in the stability. The combination of the unique properties of VHHs together with applied phage display and protein engineering is a powerful method for obtaining highly stable VHHs that can be used in a wide range of applications.
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(as it appears on PubMed at http://www.pubmed.gov), where 15640220 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15640220}}
==About this Structure==
==About this Structure==
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[[Category: Heavy chain]]
[[Category: Heavy chain]]
[[Category: Phage display]]
[[Category: Phage display]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:47:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:51:38 2008''

Revision as of 15:51, 27 July 2008

Image:1sjx.png

Template:STRUCTURE 1sjx

Three-Dimensional Structure of a Llama VHH Domain OE7 binding the cell wall protein Malf1

Template:ABSTRACT PUBMED 15640220

About this Structure

Full crystallographic information is available from OCA.

Reference

Isolation of llama antibody fragments for prevention of dandruff by phage display in shampoo., Dolk E, van der Vaart M, Lutje Hulsik D, Vriend G, de Haard H, Spinelli S, Cambillau C, Frenken L, Verrips T, Appl Environ Microbiol. 2005 Jan;71(1):442-50. PMID:15640220

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