1slu

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[[Image:1slu.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1slu| PDB=1slu | SCENE= }}
{{STRUCTURE_1slu| PDB=1slu | SCENE= }}
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'''RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN'''
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===RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN===
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==Overview==
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The three-dimensional structures of complexes of trypsin N143H, E151H bound to ecotin A86H are determined at 2.0 A resolution via X-ray crystallography in the absence and presence of the transition metals Zn2+, Ni2+, and Cu2+. The binding site for these transition metals was constructed by substitution of key amino acids with histidine at the trypsin-ecotin interface in the S2'/P2' pocket. Three histidine side chains, two on trypsin at positions 143 and 151 and one on ecotin at position 86, anchor the metals and provide extended catalytic recognition for substrates with His in the P2' pocket. Comparisons of the three-dimensional structures show the different geometries that result upon the binding of metal in the engineered tridentate site and suggest a structural basis for the kinetics of the metal-regulated catalysis. Of the three metals, the binding of zinc results in the most favorable binding geometry, not dissimilar to those observed in naturally occurring zinc binding proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 8634241 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8634241}}
==About this Structure==
==About this Structure==
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[[Category: Protein engineering]]
[[Category: Protein engineering]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:51:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:26:53 2008''

Revision as of 20:27, 27 July 2008

Image:1slu.png

Template:STRUCTURE 1slu

RAT ANIONIC N143H, E151H TRYPSIN COMPLEXED TO A86H ECOTIN

Template:ABSTRACT PUBMED 8634241

About this Structure

1SLU is a Protein complex structure of sequences from Escherichia coli and Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

X-ray structures of a designed binding site in trypsin show metal-dependent geometry., Brinen LS, Willett WS, Craik CS, Fletterick RJ, Biochemistry. 1996 May 14;35(19):5999-6009. PMID:8634241

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