1w9c
From Proteopedia
(New page: 200px<br /> <applet load="1w9c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w9c, resolution 2.30Å" /> '''PROTEOLYTIC FRAGMEN...) |
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| - | [[Image:1w9c. | + | [[Image:1w9c.jpg|left|200px]]<br /><applet load="1w9c" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1w9c, resolution 2.30Å" /> | caption="1w9c, resolution 2.30Å" /> | ||
'''PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS'''<br /> | '''PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W9C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1W9C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W9C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: nuclear protein]] | [[Category: nuclear protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:05:38 2008'' |
Revision as of 15:05, 15 February 2008
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PROTEOLYTIC FRAGMENT OF CRM1 SPANNING SIX C-TERMINAL HEAT REPEATS
Overview
CRM1/Exportin1 mediates the nuclear export of proteins bearing a, leucine-rich nuclear export signal (NES) by forming a cooperative ternary, complex with the NES-bearing substrate and the small GTPase Ran. We, present a structural model of human CRM1 based on a combination of X-ray, crystallography, homology modeling, and electron microscopy. The, architecture of CRM1 resembles that of the import receptor transportin1, with 19 HEAT repeats and a large loop implicated in Ran binding. Residues, critical for NES recognition are identified adjacent to the cysteine, residue targeted by leptomycin B (LMB), a specific CRM1 inhibitor. We, present evidence that a conformational change of the Ran binding loop, accounts for the cooperativity of Ran- and substrate binding and for the, selective enhancement of CRM1-mediated export by the cofactor RanBP3. Our, findings indicate that a single architectural and mechanistic framework, can explain the divergent effects of RanGTP on substrate binding by many, import and export receptors.
About this Structure
1W9C is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex., Petosa C, Schoehn G, Askjaer P, Bauer U, Moulin M, Steuerwald U, Soler-Lopez M, Baudin F, Mattaj IW, Muller CW, Mol Cell. 2004 Dec 3;16(5):761-75. PMID:15574331
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