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1srq

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{{STRUCTURE_1srq| PDB=1srq | SCENE= }}
{{STRUCTURE_1srq| PDB=1srq | SCENE= }}
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'''Crystal Structure of the Rap1GAP catalytic domain'''
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===Crystal Structure of the Rap1GAP catalytic domain===
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==Overview==
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Rap1 is a Ras-like guanine-nucleotide-binding protein (GNBP) that is involved in a variety of signal-transduction processes. It regulates integrin-mediated cell adhesion and might activate extracellular signal-regulated kinase. Like other Ras-like GNBPs, Rap1 is regulated by guanine-nucleotide-exchange factors (GEFs) and GTPase-activating proteins (GAPs). These GAPs increase the slow intrinsic GTPase reaction of Ras-like GNBPs by many orders of magnitude and allow tight regulation of signalling. The activation mechanism involves stabilization of the catalytic glutamine of the GNBP and, in most cases, the insertion of a catalytic arginine of GAP into the active site. Rap1 is a close homologue of Ras but does not possess the catalytic glutamine essential for GTP hydrolysis in all other Ras-like and Galpha proteins. Furthermore, RapGAPs are not related to other GAPs and apparently do not use a catalytic arginine residue. Here we present the crystal structure of the catalytic domain of the Rap1-specific Rap1GAP at 2.9 A. By mutational analysis, fluorescence titration and stopped-flow kinetic assay, we demonstrate that Rap1GAP provides a catalytic asparagine to stimulate GTP hydrolysis. Implications for the disease tuberous sclerosis are discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 15141215 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15141215}}
==About this Structure==
==About this Structure==
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[[Category: Wittinghofer, A.]]
[[Category: Wittinghofer, A.]]
[[Category: Mixed alpha-beta]]
[[Category: Mixed alpha-beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:04:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:22:26 2008''

Revision as of 16:22, 28 July 2008

Image:1srq.png

Template:STRUCTURE 1srq

Crystal Structure of the Rap1GAP catalytic domain

Template:ABSTRACT PUBMED 15141215

About this Structure

1SRQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The GTPase-activating protein Rap1GAP uses a catalytic asparagine., Daumke O, Weyand M, Chakrabarti PP, Vetter IR, Wittinghofer A, Nature. 2004 May 13;429(6988):197-201. PMID:15141215

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