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1sth

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{{STRUCTURE_1sth| PDB=1sth | SCENE= }}
{{STRUCTURE_1sth| PDB=1sth | SCENE= }}
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'''TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES'''
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===TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES===
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==Overview==
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Two crystal structures of ternary complexes of staphylococcal nuclease, cobalt(II), and the mononucleotide pdTp are reported. The first has been refined at 1.7 A to a crystallographic R value of 0.198; the second, determined from a crystal soaked for 9 months in a slightly different mother liquor than the first crystal, has been refined at 1.85 A to an R value of 0.174. In the first structure, the cobalt ion is displaced 1.94 A from the normal calcium position, and the active site is dominated by a salt bridge between Asp-21 and Lys-70 from a symmetry-related molecule in the crystal lattice. The Co2+ ion appears unable to displace this lysine; consequently, the metal is bound in a vestibular site adjacent to the calcium site. The metal-binding pocket in the second structure adopts a configuration similar to that of the calcium complex, with the cobalt ion binding only 0.36 A from the calcium position. However, an inner sphere water seen in the calcium structure is missing from this structure. The cobalt ion in the second structure appears to be loosely or transiently coordinated within the calcium binding pocket, as evidenced by the high value of its refined thermal factor. Loss of catalytic activity for cobalt(II)-substituted nuclease is perhaps due to its inability to bind this inner sphere water.
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(as it appears on PubMed at http://www.pubmed.gov), where 7703245 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7703245}}
==About this Structure==
==About this Structure==
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[[Category: Loll, P J.]]
[[Category: Loll, P J.]]
[[Category: Quirk, S.]]
[[Category: Quirk, S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:07:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:24:10 2008''

Revision as of 10:24, 27 July 2008

Image:1sth.png

Template:STRUCTURE 1sth

TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES

Template:ABSTRACT PUBMED 7703245

About this Structure

1STH is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of staphylococcal nuclease complexed with the competitive inhibitor cobalt(II) and nucleotide., Loll PJ, Quirk S, Lattman EE, Garavito RM, Biochemistry. 1995 Apr 4;34(13):4316-24. PMID:7703245

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