1sxt

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1sxt.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1sxt.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1sxt| PDB=1sxt | SCENE= }}
{{STRUCTURE_1sxt| PDB=1sxt | SCENE= }}
-
'''STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC'''
+
===STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC===
-
==Overview==
+
<!--
-
Superantigens form complexes with major histocompatibility complex (MHC) class II molecules and T-cell receptors resulting in extremely strong immunostimulatory properties. Staphylococcus aureus enterotoxin A (SEA) belongs to a subgroup of the staphylococcal superantigens that utilizes Zn2+ in the high affinity interaction with MHC class II molecules. A high affinity metal binding site was described previously in SEA co-crystallized with Cd2+ in which the metal ion was octahedrally co-ordinated, involving the N-terminal serine. We have now co-crystallized SEA with its native co-factor Zn2+ and determined its crystal structure at 2.7 A resolution. As expected for a Zn2+ ion, the co-ordination was found to be tetrahedral. Three of the ligands are located on the SEA surface on a C-terminal domain beta-sheet, while the fourth varies with the conditions. Further analysis of the zinc binding event was performed using titration microcalorimetry, which showed that SEA binds Zn2+ with an affinity of KD = 0.3 microM in an entropy driven process. The differential Zn2+ co-ordination observed here has implications for the mechanism of the SEA-MHC class II interaction.
+
The line below this paragraph, {{ABSTRACT_PUBMED_8943278}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8943278 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8943278}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Superantigen]]
[[Category: Superantigen]]
[[Category: Toxin]]
[[Category: Toxin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:15:38 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:03:00 2008''

Revision as of 18:03, 28 July 2008

Image:1sxt.png

Template:STRUCTURE 1sxt

STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC

Template:ABSTRACT PUBMED 8943278

About this Structure

1SXT is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The Co-crystal structure of staphylococcal enterotoxin type A with Zn2+ at 2.7 A resolution. Implications for major histocompatibility complex class II binding., Sundstrom M, Hallen D, Svensson A, Schad E, Dohlsten M, Abrahmsen L, J Biol Chem. 1996 Dec 13;271(50):32212-6. PMID:8943278

Page seeded by OCA on Mon Jul 28 21:03:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sxt"
Personal tools