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1sz1

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{{STRUCTURE_1sz1| PDB=1sz1 | SCENE= }}
{{STRUCTURE_1sz1| PDB=1sz1 | SCENE= }}
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'''Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes'''
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===Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes===
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==Overview==
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Transfer RNA nucleotidyltransferases (CCA-adding enzymes) are responsible for the maturation or repair of the functional 3' end of tRNAs by means of the addition of the essential nucleotides CCA. However, it is unclear how tRNA nucleotidyltransferases polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. Here we describe the crystal structure of the Archaeoglobus fulgidus tRNA nucleotidyltransferase in complex with tRNA. We also present ternary complexes of this enzyme with both RNA duplex mimics of the tRNA acceptor stem that terminate with the nucleotides C74 or C75, as well as the appropriate incoming nucleoside 5'-triphosphates. A single nucleotide-binding pocket exists whose specificity for both CTP and ATP is determined by the protein side chain of Arg 224 and backbone phosphates of the tRNA, which are non-complementary to and thus exclude UTP and GTP. Discrimination between CTP or ATP at a given addition step and at termination arises from changes in the size and shape of the nucleotide binding site that is progressively altered by the elongating 3' end of the tRNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_15295590}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15295590 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15295590}}
==About this Structure==
==About this Structure==
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[[Category: Specificity]]
[[Category: Specificity]]
[[Category: Trna]]
[[Category: Trna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:17:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:34:11 2008''

Revision as of 16:34, 28 July 2008

Image:1sz1.png

Template:STRUCTURE 1sz1

Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes

Template:ABSTRACT PUBMED 15295590

About this Structure

1SZ1 is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template., Xiong Y, Steitz TA, Nature. 2004 Aug 5;430(7000):640-5. PMID:15295590

Page seeded by OCA on Mon Jul 28 19:34:11 2008

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