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| - | [[Image:1szn.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1szn.png|left|200px]] |
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| | {{STRUCTURE_1szn| PDB=1szn | SCENE= }} | | {{STRUCTURE_1szn| PDB=1szn | SCENE= }} |
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| - | '''THE STRUCTURE OF ALPHA-GALACTOSIDASE'''
| + | ===THE STRUCTURE OF ALPHA-GALACTOSIDASE=== |
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| - | ==Overview==
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| - | The crystal structures of alpha-galactosidase from the mesophilic fungus Trichoderma reesei and its complex with the competitive inhibitor, beta-d-galactose, have been determined at 1.54 A and 2.0 A resolution, respectively. The alpha-galactosidase structure was solved by the quick cryo-soaking method using a single Cs derivative. The refined crystallographic model of the alpha-galactosidase consists of two domains, an N-terminal catalytic domain of the (beta/alpha)8 barrel topology and a C-terminal domain which is formed by an antiparallel beta-structure. The protein contains four N-glycosylation sites located in the catalytic domain. Some of the oligosaccharides were found to participate in inter-domain contacts. The galactose molecule binds to the active site pocket located in the center of the barrel of the catalytic domain. Analysis of the alpha-galactosidase- galactose complex reveals the residues of the active site and offers a structural basis for identification of the putative mechanism of the enzymatic reaction. The structure of the alpha-galactosidase closely resembles those of the glycoside hydrolase family 27. The conservation of two catalytic Asp residues, identified for this family, is consistent with a double-displacement reaction mechanism for the alpha-galactosidase. Modeling of possible substrates into the active site reveals specific hydrogen bonds and hydrophobic interactions that could explain peculiarities of the enzyme kinetics. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15136043}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15136043 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15136043}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ev, A N.Savel.]] | | [[Category: Ev, A N.Savel.]] |
| | [[Category: Glycoprotein]] | | [[Category: Glycoprotein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:19:05 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:58:39 2008'' |
Revision as of 08:58, 29 July 2008
Template:STRUCTURE 1szn
THE STRUCTURE OF ALPHA-GALACTOSIDASE
Template:ABSTRACT PUBMED 15136043
About this Structure
1SZN is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.
Reference
Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism., Golubev AM, Nagem RA, Brandao Neto JR, Neustroev KN, Eneyskaya EV, Kulminskaya AA, Shabalin KA, Savel'ev AN, Polikarpov I, J Mol Biol. 2004 May 28;339(2):413-22. PMID:15136043
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