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1t1o

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{{STRUCTURE_1t1o| PDB=1t1o | SCENE= }}
{{STRUCTURE_1t1o| PDB=1t1o | SCENE= }}
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'''Components of the control 70S ribosome to provide reference for the RRF binding site'''
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===Components of the control 70S ribosome to provide reference for the RRF binding site===
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==Overview==
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After the termination step of protein synthesis, a deacylated tRNA and mRNA remain associated with the ribosome. The ribosome-recycling factor (RRF), together with elongation factor G (EF-G), disassembles this posttermination complex into mRNA, tRNA, and the ribosome. We have obtained a three-dimensional cryo-electron microscopic map of a complex of the Escherichia coli 70S ribosome and RRF. We find that RRF interacts mainly with the segments of the large ribosomal subunit's (50S) rRNA helices that are involved in the formation of two central intersubunit bridges, B2a and B3. The binding of RRF induces considerable conformational changes in some of the functional domains of the ribosome. As compared to its binding position derived previously by hydroxyl radical probing study, we find that RRF binds further inside the intersubunit space of the ribosome such that the tip of its domain I is shifted (by approximately 13 A) toward protein L5 within the central protuberance of the 50S subunit, and domain II is oriented more toward the small ribosomal subunit (30S). Overlapping binding sites of RRF, EF-G, and the P-site tRNA suggest that the binding of EF-G would trigger the removal of deacylated tRNA from the P site by moving RRF toward the ribosomal E site, and subsequent removal of mRNA may be induced by a shift in the position of 16S rRNA helix 44, which harbors part of the mRNA.
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(as it appears on PubMed at http://www.pubmed.gov), where 15178758 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15178758}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications., Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J, Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8900-5. Epub 2004 Jun 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15178758 15178758]
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications., Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J, Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8900-5. Epub 2004 Jun 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15178758 15178758]
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High resolution structure of the large ribosomal subunit from a mesophilic eubacterium., Harms J, Schluenzen F, Zarivach R, Bashan A, Gat S, Agmon I, Bartels H, Franceschi F, Yonath A, Cell. 2001 Nov 30;107(5):679-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11733066 11733066]
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Structure of the 30S ribosomal subunit., Wimberly BT, Brodersen DE, Clemons WM Jr, Morgan-Warren RJ, Carter AP, Vonrhein C, Hartsch T, Ramakrishnan V, Nature. 2000 Sep 21;407(6802):327-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11014182 11014182]
[[Category: Agrawal, R K.]]
[[Category: Agrawal, R K.]]
[[Category: Booth, T M.]]
[[Category: Booth, T M.]]
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[[Category: Spahn, C M.]]
[[Category: Spahn, C M.]]
[[Category: Rrf binding position on the ribosome]]
[[Category: Rrf binding position on the ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:23:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:42:43 2008''

Revision as of 11:42, 28 July 2008

Image:1t1o.png

Template:STRUCTURE 1t1o

Components of the control 70S ribosome to provide reference for the RRF binding site

Template:ABSTRACT PUBMED 15178758

About this Structure

Full crystallographic information is available from OCA.

Reference

Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications., Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J, Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8900-5. Epub 2004 Jun 3. PMID:15178758

High resolution structure of the large ribosomal subunit from a mesophilic eubacterium., Harms J, Schluenzen F, Zarivach R, Bashan A, Gat S, Agmon I, Bartels H, Franceschi F, Yonath A, Cell. 2001 Nov 30;107(5):679-88. PMID:11733066

Structure of the 30S ribosomal subunit., Wimberly BT, Brodersen DE, Clemons WM Jr, Morgan-Warren RJ, Carter AP, Vonrhein C, Hartsch T, Ramakrishnan V, Nature. 2000 Sep 21;407(6802):327-39. PMID:11014182

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