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1t4x

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{{STRUCTURE_1t4x| PDB=1t4x | SCENE= }}
{{STRUCTURE_1t4x| PDB=1t4x | SCENE= }}
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'''The first left-handed RNA structure of (CGCGCG)2, Z-RNA, NMR, 12 structures, determined in high salt'''
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===The first left-handed RNA structure of (CGCGCG)2, Z-RNA, NMR, 12 structures, determined in high salt===
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==Overview==
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Right-handed RNA duplexes of (CG)n sequence undergo salt-induced helicity reversal, forming left-handed RNA double helices (Z-RNA). In contrast to the thoroughly studied Z-DNA, no Z-RNA structure of natural origin is known. Here we report the NMR structure of a half-turn, left-handed RNA helix (CGCGCG)2 determined in 6 M NaClO4. This is the first nucleic acid motif determined at such high salt. Sequential assignments of non-exchangeable proton resonances of the Z-form were based on the hitherto unreported NOE connectivity path [H6(n)-H5'/H5''(n)-H8(n+1)-H1'(n+1)-H6(n+2)] found for left-handed helices. Z-RNA structure shows several conformational features significantly different from Z-DNA. Intra-strand but no inter-strand base stacking was observed for both CpG and GpC steps. Helical twist angles for CpG steps have small positive values (4-7 degrees), whereas GpC steps have large negative values (-61 degrees). In the full-turn model of Z-RNA (12.4 bp per turn), base pairs are much closer to the helix axis than in Z-DNA, thus both the very deep, narrow minor groove with buried cytidine 2'-OH groups, and the major groove are well defined. The 2'-OH group of cytidines plays a crucial role in the Z-RNA structure and its formation; 2'-O-methylation of cytidine, but not of guanosine residues prohibits A to Z helicity reversal.
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(as it appears on PubMed at http://www.pubmed.gov), where 15292450 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15292450}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4X OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T4X OCA].
==Reference==
==Reference==
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[[Category: Left-handed rna duplex]]
[[Category: Left-handed rna duplex]]
[[Category: Z-rna]]
[[Category: Z-rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:32:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:17:41 2008''

Revision as of 04:17, 28 July 2008

Image:1t4x.png

Template:STRUCTURE 1t4x

The first left-handed RNA structure of (CGCGCG)2, Z-RNA, NMR, 12 structures, determined in high salt

Template:ABSTRACT PUBMED 15292450

About this Structure

Full experimental information is available from OCA.

Reference

High salt solution structure of a left-handed RNA double helix., Popenda M, Milecki J, Adamiak RW, Nucleic Acids Res. 2004 Aug 3;32(13):4044-54. Print 2004. PMID:15292450

Page seeded by OCA on Mon Jul 28 07:17:41 2008

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