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1t66

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[[Image:1t66.gif|left|200px]]
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{{STRUCTURE_1t66| PDB=1t66 | SCENE= }}
{{STRUCTURE_1t66| PDB=1t66 | SCENE= }}
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'''The structure of FAB with intermediate affinity for fluorescein.'''
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===The structure of FAB with intermediate affinity for fluorescein.===
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==Overview==
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Multi-disciplinary studies of fluorescein-protein conjugates have led to the generation of a family of antibodies with common idiotypes and affinities for fluorescein ranging over five orders of magnitude. The high affinity 4-4-20 prototype traps the ligand in a highly complementary binding slot, which is lined by multiple aromatic side-chains. An antibody (9-40) of intermediate affinity belongs to the same idiotypic family as 4-4-20 and shares substantial amino acid identities within the VL and VH domains. To establish the structural basis for the affinity differences, we solved the crystal structure of the 9-40 Fab-fluorescein complex at a resolution of 2.3A. Similar to 4-4-20, 9-40 binds fluorescein in a tight aromatic slot with its xanthenonyl ring system accommodated by end-on insertion. However, the combined effects of the amino acid substitutions have resulted in reorganization of the binding site, with the HCDR3 loops showing the greatest differences in conformations. Access to the binding site of 9-40 is substantially more open, leaving the fluorescein's phenylcarboxylate moiety partially exposed to solvent. In addition to the usage of a different D (diversity) mini-gene encoding the HCDR3 loop, the decrease in fluorescein affinity in the 9-40 antibody family appears to be correlated with the substitution of histidine (9-40) for arginine (4-4-20) in position 34 of the antibody light chains.
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(as it appears on PubMed at http://www.pubmed.gov), where 15178254 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15178254}}
==About this Structure==
==About this Structure==
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[[Category: Fab]]
[[Category: Fab]]
[[Category: Fluorescein]]
[[Category: Fluorescein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:34:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:13:04 2008''

Revision as of 08:13, 28 July 2008

Image:1t66.png

Template:STRUCTURE 1t66

The structure of FAB with intermediate affinity for fluorescein.

Template:ABSTRACT PUBMED 15178254

About this Structure

1T66 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Three-dimensional structures of idiotypically related Fabs with intermediate and high affinity for fluorescein., Terzyan S, Ramsland PA, Voss EW Jr, Herron JN, Edmundson AB, J Mol Biol. 2004 Jun 18;339(5):1141-51. PMID:15178254

Page seeded by OCA on Mon Jul 28 11:13:04 2008

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