We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1t8k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1t8k.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1t8k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1t8k| PDB=1t8k | SCENE= }}
{{STRUCTURE_1t8k| PDB=1t8k | SCENE= }}
-
'''Crystal Structure of apo acyl carrier protein from E. coli'''
+
===Crystal Structure of apo acyl carrier protein from E. coli===
-
==Overview==
+
<!--
-
A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15333924}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15333924 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15333924}}
==About this Structure==
==About this Structure==
Line 25: Line 29:
[[Category: Qiu, X.]]
[[Category: Qiu, X.]]
[[Category: Acp]]
[[Category: Acp]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:40:11 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:51:24 2008''

Revision as of 11:51, 29 July 2008

Image:1t8k.png

Template:STRUCTURE 1t8k

Crystal Structure of apo acyl carrier protein from E. coli

Template:ABSTRACT PUBMED 15333924

About this Structure

1T8K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions., Qiu X, Janson CA, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924

Page seeded by OCA on Tue Jul 29 14:51:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t8k"
Personal tools