9d5n
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==48-nm doublet microtubule from Trichomonas vaginalis strain G3== | |
| + | <StructureSection load='9d5n' size='340' side='right'caption='[[9d5n]], [[Resolution|resolution]] 4.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9d5n]] is a 215 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichomonas_vaginalis_G3 Trichomonas vaginalis G3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9D5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9D5N FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9d5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9d5n OCA], [https://pdbe.org/9d5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9d5n RCSB], [https://www.ebi.ac.uk/pdbsum/9d5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9d5n ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A2FZ95_TRIV3 A2FZ95_TRIV3] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Doublet microtubules (DMTs) are flagellar components required for the protist Trichomonas vaginalis (Tv) to swim through the human genitourinary tract to cause trichomoniasis, the most common non-viral sexually transmitted disease. Lack of structures of Tv's DMT (Tv-DMT) has prevented structure-guided drug design to manage Tv infection. Here, we determine the 16 nm, 32 nm, 48 nm and 96 nm-repeat structures of native Tv-DMT at resolution ranging from 3.4 to 4.4 A by cryogenic electron microscopy (cryoEM) and built an atomic model for the entire Tv-DMT. These structures show that Tv-DMT is composed of 30 different proteins, including the alpha- and beta-tubulin, 19 microtubule inner proteins (MIPs) and 9 microtubule outer proteins. While the A-tubule of Tv-DMT is simplistic compared to DMTs of other organisms, the B-tubule of Tv-DMT features parasite-specific proteins, such as TvFAP40 and TvFAP35. Notably, TvFAP40 and TvFAP35 form filaments near the inner and outer junctions, respectively, and interface with stabilizing MIPs. This atomic model of the Tv-DMT highlights diversity of eukaryotic motility machineries and provides a structural framework to inform rational design of therapeutics against trichomoniasis. | ||
| - | + | Structures of Native Doublet Microtubules from Trichomonas vaginalis Reveal Parasite-Specific Proteins.,Stevens A, Kashyap S, Crofut EH, Wang SE, Muratore KA, Johnson PJ, Zhou ZH Nat Commun. 2025 Apr 29;16(1):3996. doi: 10.1038/s41467-025-59369-y. PMID:40301421<ref>PMID:40301421</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9d5n" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Trichomonas vaginalis G3]] | ||
| + | [[Category: Crofut EJ]] | ||
| + | [[Category: Kashyap S]] | ||
| + | [[Category: Stevens A]] | ||
| + | [[Category: Zhou HZ]] | ||
Current revision
48-nm doublet microtubule from Trichomonas vaginalis strain G3
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