1t92

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{{STRUCTURE_1t92| PDB=1t92 | SCENE= }}
{{STRUCTURE_1t92| PDB=1t92 | SCENE= }}
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'''Crystal structure of N-terminal truncated pseudopilin PulG'''
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===Crystal structure of N-terminal truncated pseudopilin PulG===
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==Overview==
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The pseudopilin PulG is one of several essential components of the type II pullulanase secretion machinery (the Pul secreton) of the Gram-negative bacterium Klebsiella oxytoca. The sequence of the N-terminal 25 amino acids of the PulG precursor is hydrophobic and very similar to the corresponding region of type IV pilins. The structure of a truncated PulG (lacking the homologous region), as determined by X-ray crystallography, was found to include part of the long N-terminal alpha-helix and the four internal anti-parallel beta-strands that characterize type IV pilins, but PulG lacks the highly variable loop region with a disulphide bond that is found in the latter. When overproduced, PulG forms flexible pili whose structural features, as visualized by electron microscopy, are similar to those of bacterial type IV pili. The average helical repeat comprises 17 PulG subunits and four helical turns. Electron microscopy and molecular modelling show that PulG probably assembles into left-handed helical pili with the long N-terminal alpha-helix tightly packed in the centre of the pilus. As in the type IV pilins, the hydrophobic N-terminal part of the PulG alpha-helix is necessary for its assembly. Subtle sequence variations within this highly conserved segment seem to determine whether or not a type IV pilin can be assembled into pili by the Pul secreton.
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{{ABSTRACT_PUBMED_15491357}}
==About this Structure==
==About this Structure==
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[[Category: Pseudopilin]]
[[Category: Pseudopilin]]
[[Category: Zinc]]
[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:41:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:29:14 2008''

Revision as of 10:29, 27 July 2008

Image:1t92.png

Template:STRUCTURE 1t92

Crystal structure of N-terminal truncated pseudopilin PulG

Template:ABSTRACT PUBMED 15491357

About this Structure

1T92 is a Single protein structure of sequence from Klebsiella pneumoniae. Full crystallographic information is available from OCA.

Reference

Structure and assembly of the pseudopilin PulG., Kohler R, Schafer K, Muller S, Vignon G, Diederichs K, Philippsen A, Ringler P, Pugsley AP, Engel A, Welte W, Mol Microbiol. 2004 Nov;54(3):647-64. PMID:15491357

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