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1t9e

From Proteopedia

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[[Image:1t9e.gif|left|200px]]
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[[Image:1t9e.png|left|200px]]
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{{STRUCTURE_1t9e| PDB=1t9e | SCENE= }}
{{STRUCTURE_1t9e| PDB=1t9e | SCENE= }}
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'''NMR solution structure of a disulfide analogue of the cyclic sunflower trypsin inhibitor SFTI-1'''
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===NMR solution structure of a disulfide analogue of the cyclic sunflower trypsin inhibitor SFTI-1===
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==Overview==
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SFTI-1 is a novel 14 amino acid peptide comprised of a circular backbone constrained by three proline residues, a hydrogen-bond network, and a single disulfide bond. It is the smallest and most potent known Bowman-Birk trypsin inhibitor and the only one with a cyclic peptidic backbone. The solution structure of [ABA(3,11)]SFTI-1, a disulfide-deficient analogue of SFTI-1, has been determined by (1)H NMR spectroscopy. The lowest energy structures of native SFTI-1 and [ABA(3,11)]SFTI-1 are similar and superimpose with a root-mean-square deviation over the backbone and heavy atoms of 0.26 +/- 0.09 and 1.10 +/- 0.22 A, respectively. The disulfide bridge in SFTI-1 was found to be a minor determinant for the overall structure, but its removal resulted in a slightly weakened hydrogen-bonding network. To further investigate the role of the disulfide bridge, NMR chemical shifts for the backbone H(alpha) protons of two disulfide-deficient linear analogues of SFTI-1, [ABA(3,11)]SFTI-1[6,5] and [ABA(3,11)]SFTI-1[1,14] were measured. These correspond to analogues of the cleavage product of SFTI-1 and a putative biosynthetic precursor, respectively. In contrast with the cyclic peptide, it was found that the disulfide bridge is essential for maintaining the structure of these open-chain analogues. Overall, the hydrogen-bond network appears to be a crucial determinant of the structure of SFTI-1 analogues.
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The line below this paragraph, {{ABSTRACT_PUBMED_15667208}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15667208 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15667208}}
==About this Structure==
==About this Structure==
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1T9E is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T9E OCA].
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1T9E is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T9E OCA].
==Reference==
==Reference==
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Sunflower trypsin inhibitor]]
[[Category: Sunflower trypsin inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:42:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:12:06 2008''

Revision as of 07:12, 29 July 2008

Image:1t9e.png

Template:STRUCTURE 1t9e

NMR solution structure of a disulfide analogue of the cyclic sunflower trypsin inhibitor SFTI-1

Template:ABSTRACT PUBMED 15667208

About this Structure

1T9E is a Single protein structure. Full experimental information is available from OCA.

Reference

Disulfide bond mutagenesis and the structure and function of the head-to-tail macrocyclic trypsin inhibitor SFTI-1., Korsinczky ML, Clark RJ, Craik DJ, Biochemistry. 2005 Feb 1;44(4):1145-53. PMID:15667208

Page seeded by OCA on Tue Jul 29 10:12:06 2008

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