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1taq

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{{STRUCTURE_1taq| PDB=1taq | SCENE= }}
{{STRUCTURE_1taq| PDB=1taq | SCENE= }}
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'''STRUCTURE OF TAQ DNA POLYMERASE'''
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===STRUCTURE OF TAQ DNA POLYMERASE===
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==Overview==
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The DNA polymerase from Thermus aquaticus (Taq polymerase), famous for its use in the polymerase chain reaction, is homologous to Escherichia coli DNA polymerase I (pol I) Like pol I, Taq polymerase has a domain at its amino terminus (residues 1-290) that has 5' nuclease activity and a domain at its carboxy terminus that catalyses the polymerase reaction. Unlike pol I, the intervening domain in Taq polymerase has lost the editing 3'-5' exonuclease activity. Although the structure of the Klenow fragment of pol I has been known for ten years, that of the intact pol I has proved more elusive. The structure of Taq polymerase determined here at 2.4 A resolution shows that the structures of the polymerase domains of the thermostable enzyme and of the Klenow fragment are nearly identical, whereas the catalytically critical carboxylate residues that bind two metal ions are missing from the remnants of the 3'-5' exonuclease active site of Taq polymerase. The first view of the 5' nuclease domain, responsible for excising the Okazaki RNA in lagging-strand DNA replication, shows a cluster of conserved divalent metal-ion-binding carboxylates at the bottom of a cleft. The location of this 5'-nuclease active site some 70 A from the polymerase active site in this crystal form highlights the unanswered question of how this domain works in concert with the polymerase domain to produce a duplex DNA product that contains only a nick.
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(as it appears on PubMed at http://www.pubmed.gov), where 7637814 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7637814}}
==About this Structure==
==About this Structure==
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[[Category: Pcr polymerase]]
[[Category: Pcr polymerase]]
[[Category: Taq dna polymerase]]
[[Category: Taq dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:44:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:59:28 2008''

Revision as of 10:59, 27 July 2008

Image:1taq.png

Template:STRUCTURE 1taq

STRUCTURE OF TAQ DNA POLYMERASE

Template:ABSTRACT PUBMED 7637814

About this Structure

1TAQ is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.

Reference

Crystal structure of Thermus aquaticus DNA polymerase., Kim Y, Eom SH, Wang J, Lee DS, Suh SW, Steitz TA, Nature. 1995 Aug 17;376(6541):612-6. PMID:7637814

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