1tau

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[[Image:1tau.gif|left|200px]]
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==Overview==
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{{ABSTRACT_8717047}}
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The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a vestigial editing 3'-5' exonuclease domain of Taq polymerase that lies between the other two domains is dramatically altered, resulting in the absence of this activity in the thermostable enzyme. The structures have been solved for editing complexes between KF and single-stranded DNA and for duplex DNA with a 3' overhanging single strand, but not for a complex containing duplex DNA at the polymerase active-site. Here we present the co-crystal structure of Taq polymerase with a blunt-ended duplex DNA bound to the polymerase active-site cleft; the DNA neither bends nor goes through the large polymerase cleft, and the structural form of the bound DNA is between the B and A forms. A wide minor groove allows access to protein side chains that hydrogen-bond to the N3 of purines and the O2 of pyrimidines at the blunt-end terminus. Part of the DNA bound to the polymerase site shares a common binding site with DNA bound to the exonuclease site, but they are translated relative to each other by several angstroms along their helix axes.
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==About this Structure==
==About this Structure==
1TAU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. The following page contains interesting information on the relation of 1TAU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb3_1.html DNA Polymerase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAU OCA].
1TAU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. The following page contains interesting information on the relation of 1TAU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb3_1.html DNA Polymerase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TAU OCA].
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==Reference==
 
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Structure of Taq polymerase with DNA at the polymerase active site., Eom SH, Wang J, Steitz TA, Nature. 1996 Jul 18;382(6588):278-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8717047 8717047]
 
[[Category: DNA Polymerase]]
[[Category: DNA Polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
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[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Taq dna polymerase]]
[[Category: Taq dna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:45:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:59:38 2008''

Revision as of 14:59, 26 June 2008

Image:1tau.png

Template:STRUCTURE 1tau

TAQ POLYMERASE (E.C.2.7.7.7)/DNA/B-OCTYLGLUCOSIDE COMPLEX


Template:ABSTRACT 8717047

About this Structure

1TAU is a Single protein structure of sequence from Thermus aquaticus. The following page contains interesting information on the relation of 1TAU with [DNA Polymerase]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:59:38 2008

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