1td4

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{{STRUCTURE_1td4| PDB=1td4 | SCENE= }}
{{STRUCTURE_1td4| PDB=1td4 | SCENE= }}
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'''Crystal structure of VSHP_BPP21 in space group H3 with high resolution.'''
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===Crystal structure of VSHP_BPP21 in space group H3 with high resolution.===
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==Overview==
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SHP, the capsid-stabilizing protein of lambdoid phage 21, is highly resistant against denaturant-induced unfolding. We demonstrate that this high functional stability of SHP is due to a high kinetic stability with a half-life for unfolding of 25 days at zero denaturant, while the thermodynamic stability is not unusually high. Unfolding experiments demonstrated that the trimeric state (also observed in crystals and present on the phage capsid) of SHP is kinetically stable in solution, while the monomer intermediate unfolds very rapidly. We also determined the crystal structure of trimeric SHP at 1.5A resolution, which was compared to that of its functional homolog gpD. This explains how a tight network of H-bonds rigidifies crucial interpenetrating residues, leading to the observed extremely slow trimer dissociation or denaturation. Taken as a whole, our results provide molecular-level insights into natural strategies to achieve kinetic stability by taking advantage of protein oligomerization. Kinetic stability may be especially needed in phage capsids to allow survival in harsh environments.
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{{ABSTRACT_PUBMED_15504410}}
==About this Structure==
==About this Structure==
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[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
[[Category: Shp]]
[[Category: Shp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:48:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:01:04 2008''

Revision as of 18:01, 27 July 2008

Image:1td4.png

Template:STRUCTURE 1td4

Crystal structure of VSHP_BPP21 in space group H3 with high resolution.

Template:ABSTRACT PUBMED 15504410

About this Structure

1TD4 is a Single protein structure of sequence from Enterobacteria phage p21. Full crystallographic information is available from OCA.

Reference

Kinetic stability and crystal structure of the viral capsid protein SHP., Forrer P, Chang C, Ott D, Wlodawer A, Pluckthun A, J Mol Biol. 2004 Nov 12;344(1):179-93. PMID:15504410

Page seeded by OCA on Sun Jul 27 21:01:04 2008

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