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1tio

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[[Image:1tio.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1tio| PDB=1tio | SCENE= }}
{{STRUCTURE_1tio| PDB=1tio | SCENE= }}
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'''HIGH PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE'''
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===HIGH PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE===
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==Overview==
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Two orthorhombic forms (Vm values are 2.3 and 3.0 A3/Da) of bovine beta-trypsin crystals in neat cyclohexane were determined to 1.93 A resolution, by X-ray diffraction. Both structures in organic solvent are similar to those in aqueous solution. In the high packing density form, one cyclohexane molecule is found in a hydrophobic site near the active center. One sulfate locates at the active site with hydrogen or salt bond to the Ser-His catalytic diad, and five more sulfates bind on the molecular surface. The conformation of the side chains near the sulfates changed greatly. In the low packing density form, one cyclohexane and three sulfates are found. In both structures, one benzamidine molecule locates at the hydrophobic pocket of the active center. Most water molecules on the enzyme surface are retained except some with high temperature factors.
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(as it appears on PubMed at http://www.pubmed.gov), where 9920392 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9920392}}
==About this Structure==
==About this Structure==
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[[Category: Benzamidine inhibited]]
[[Category: Benzamidine inhibited]]
[[Category: Serine protease]]
[[Category: Serine protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:59:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:42:04 2008''

Revision as of 16:42, 28 July 2008

Image:1tio.png

Template:STRUCTURE 1tio

HIGH PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE

Template:ABSTRACT PUBMED 9920392

About this Structure

1TIO is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

X-ray studies on two forms of bovine beta-trypsin crystals in neat cyclohexane., Zhu G, Huang Q, Wang Z, Qian M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 Dec 8;1429(1):142-50. PMID:9920392

Page seeded by OCA on Mon Jul 28 19:42:04 2008

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