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| - | [[Image:1tjs.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1tjs| PDB=1tjs | SCENE= }} | | {{STRUCTURE_1tjs| PDB=1tjs | SCENE= }} |
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| - | '''E. COLI THYMIDYLATE SYNTHASE'''
| + | ===E. COLI THYMIDYLATE SYNTHASE=== |
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| - | ==Overview==
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| - | BACKGROUND: Enzymes have evolved to recognise their target substrates with exquisite selectivity and specificity. Whether fragments of the substrate--perhaps never available to the evolving enzyme--are bound in the same manner as the parent substrate addresses the fundamental basis of specificity. An understanding of the relative contributions of individual portions of ligand molecules to the enzyme-binding interaction may offer considerable insight into the principles of substrate recognition. RESULTS: We report 12 crystal structures of Escherichia coli thymidylate synthase in complexes with available fragments of the substrate (dUMP), both with and without the presence of a cofactor analogue. The structures display considerable fidelity of binding mode and interactions. These complexes reveal several interesting features: the cofactor analogue enhances the localisation of substrate and substrate fragments near the reactive thiol; the ribose moiety reduces local disorder through additional specific enzyme-ligand interactions; the pyrimidine has multiple roles, ranging from stereospecificity to mechanistic competence; and the glycosidic linkage has an important role in the formation of a covalent attachment between substrate and enzyme. CONCLUSIONS: The requirements of ligand-protein binding can be understood in terms of the binding of separate fragments of the ligand. Fragments which are subsystems of the natural substrate for the enzyme confer specific contributions to the binding affinity, orientation or electrostatics of the enzymatic mechanism. This ligand-binding analysis provides a complementary method to the more prevalent approaches utilising site-directed mutagenesis. In addition, these observations suggest a modular approach for rational drug design utilising chemical fragments.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9687366}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9687366 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9687366}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Stroud, R M.]] | | [[Category: Stroud, R M.]] |
| | [[Category: Substrate module]] | | [[Category: Substrate module]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:02:09 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:19:31 2008'' |
Revision as of 04:19, 28 July 2008
Template:STRUCTURE 1tjs
E. COLI THYMIDYLATE SYNTHASE
Template:ABSTRACT PUBMED 9687366
About this Structure
1TJS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The additivity of substrate fragments in enzyme-ligand binding., Stout TJ, Sage CR, Stroud RM, Structure. 1998 Jul 15;6(7):839-48. PMID:9687366
Page seeded by OCA on Mon Jul 28 07:19:31 2008
