1tku

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{{STRUCTURE_1tku| PDB=1tku | SCENE= }}
{{STRUCTURE_1tku| PDB=1tku | SCENE= }}
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'''Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate'''
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===Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate===
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==Overview==
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A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
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(as it appears on PubMed at http://www.pubmed.gov), where 15328619 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15328619}}
==About this Structure==
==About this Structure==
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[[Category: Riboflavin biosynthesis]]
[[Category: Riboflavin biosynthesis]]
[[Category: Synthetic gene]]
[[Category: Synthetic gene]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:04:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:00:49 2008''

Revision as of 09:00, 28 July 2008

Image:1tku.png

Template:STRUCTURE 1tku

Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate

Template:ABSTRACT PUBMED 15328619

About this Structure

1TKU is a Single protein structure of sequence from Candida albicans sc5314. Full crystallographic information is available from OCA.

Reference

Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619

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