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1tkw

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[[Image:1tkw.gif|left|200px]]
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{{Seed}}
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[[Image:1tkw.png|left|200px]]
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{{STRUCTURE_1tkw| PDB=1tkw | SCENE= }}
{{STRUCTURE_1tkw| PDB=1tkw | SCENE= }}
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'''The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR'''
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===The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR===
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==Overview==
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The orientation of poplar plastocyanin in the complex with turnip cytochrome f has been determined by rigid-body calculations using restraints from paramagnetic NMR measurements. The results show that poplar plastocyanin interacts with cytochrome f with the hydrophobic patch of plastocyanin close to the heme region on cytochrome f and via electrostatic interactions between the charged patches on both proteins. Plastocyanin is tilted relative to the orientation reported for spinach plastocyanin, resulting in a longer distance between iron and copper (13.9 A). With increasing ionic strength, from 0.01 to 0.11 M, all observed chemical-shift changes decrease uniformly, supporting the idea that electrostatic forces contribute to complex formation. There is no indication for a rearrangement of the transient complex in this ionic strength range, contrary to what had been proposed earlier on the basis of kinetic data. By decreasing the pH from pH 7.7 to pH 5.5, the complex is destabilized. This may be attributed to the protonation of the conserved acidic patches or the copper ligand His87 in poplar plastocyanin, which are shown to have similar pK(a) values. The results are interpreted in a two-step model for complex formation.
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(as it appears on PubMed at http://www.pubmed.gov), where 15863096 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15863096}}
==About this Structure==
==About this Structure==
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1TKW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA].
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1TKW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa] and [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKW OCA].
==Reference==
==Reference==
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[[Category: Photosynthesis]]
[[Category: Photosynthesis]]
[[Category: Rigid body calculation]]
[[Category: Rigid body calculation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:04:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:44:09 2008''

Revision as of 18:44, 27 July 2008

Image:1tkw.png

Template:STRUCTURE 1tkw

The transient complex of poplar plastocyanin with turnip cytochrome f determined with paramagnetic NMR

Template:ABSTRACT PUBMED 15863096

About this Structure

1TKW is a Protein complex structure of sequences from Brassica rapa and Populus nigra. Full experimental information is available from OCA.

Reference

The transient complex of poplar plastocyanin with cytochrome f: effects of ionic strength and pH., Lange C, Cornvik T, Diaz-Moreno I, Ubbink M, Biochim Biophys Acta. 2005 Apr-May;1707(2-3):179-88. Epub 2004 Dec 29. PMID:15863096

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