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1tr7

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{{STRUCTURE_1tr7| PDB=1tr7 | SCENE= }}
{{STRUCTURE_1tr7| PDB=1tr7 | SCENE= }}
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'''FimH adhesin receptor binding domain from uropathogenic E. coli'''
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===FimH adhesin receptor binding domain from uropathogenic E. coli===
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==Overview==
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Mannose-binding type 1 pili are important virulence factors for the establishment of Escherichia coli urinary tract infections (UTIs). These infections are initiated by adhesion of uropathogenic E. coli to uroplakin receptors in the uroepithelium via the FimH adhesin located at the tips of type 1 pili. Blocking of bacterial adhesion is able to prevent infection. Here, we provide for the first time binding data of the molecular events underlying type 1 fimbrial adherence, by crystallographic analyses of the FimH receptor binding domains from a uropathogenic and a K-12 strain, and affinity measurements with mannose, common mono- and disaccharides, and a series of alkyl and aryl mannosides. Our results illustrate that the lectin domain of the FimH adhesin is a stable and functional entity and that an exogenous butyl alpha-D-mannoside, bound in the crystal structures, exhibits a significantly better affinity for FimH (Kd = 0.15 microM) than mannose (Kd = 2.3 microM). Exploration of the binding affinities of alpha- d-mannosides with longer alkyl tails revealed affinities up to 5 nM. Aryl mannosides and fructose can also bind with high affinities to the FimH lectin domain, with a 100-fold improvement and 15-fold reduction in affinity, respectively, compared with mannose. Taken together, these relative FimH affinities correlate exceptionally well with the relative concentrations of the same glycans needed for the inhibition of adherence of type 1 piliated E. coli. We foresee that our findings will spark new ideas and initiatives for the development of UTI vaccines and anti-adhesive drugs to prevent anticipated and recurrent UTIs.
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(as it appears on PubMed at http://www.pubmed.gov), where 15659162 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15659162}}
==About this Structure==
==About this Structure==
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[[Category: Urinary tract infection]]
[[Category: Urinary tract infection]]
[[Category: Uropathogenic e. coli]]
[[Category: Uropathogenic e. coli]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:18:57 2008''

Revision as of 09:18, 29 July 2008

Image:1tr7.png

Template:STRUCTURE 1tr7

FimH adhesin receptor binding domain from uropathogenic E. coli

Template:ABSTRACT PUBMED 15659162

About this Structure

1TR7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin., Bouckaert J, Berglund J, Schembri M, De Genst E, Cools L, Wuhrer M, Hung CS, Pinkner J, Slattegard R, Zavialov A, Choudhury D, Langermann S, Hultgren SJ, Wyns L, Klemm P, Oscarson S, Knight SD, De Greve H, Mol Microbiol. 2005 Jan;55(2):441-55. PMID:15659162

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