1tre

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{{STRUCTURE_1tre| PDB=1tre | SCENE= }}
{{STRUCTURE_1tre| PDB=1tre | SCENE= }}
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'''THE STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ESCHERICHIA COLI DETERMINED AT 2.6 ANGSTROM RESOLUTION'''
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===THE STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ESCHERICHIA COLI DETERMINED AT 2.6 ANGSTROM RESOLUTION===
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==Overview==
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The structure of triosephosphate isomerase (TIM) from the organism Escherichia coli has been determined at a resolution of 2.6 A. The structure was solved by the molecular replacement method, first at 2.8 A resolution with a crystal grown by the technique of hanging-drop crystallization from a mother liquor containing the transition-state analogue 2-phosphoglycolate (2PG). As a search model in the molecular replacement calculations, the refined structure of TIM from Trypanosoma brucei, which has a sequence identity of 46% compared to the enzyme from E. coli, was used. An E. coli TIM crystal grown in the absence of 2PG, diffracting to 2.6 A resolution, was later obtained by application of the technique of macro-seeding using a seed crystal grown from a mother liquor without 2PG. The final 2.6 A model has a crystallographic R factor of 11.9%, and agrees well with standard stereochemical parameters. The structure of E. coli TIM suggests the importance of residues which favour helix initiation for the formation of the TIM fold. In addition, TIM from E. coli shows peculiarities in its dimer interface, and in the packing of core residues within the beta-barrel.
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(as it appears on PubMed at http://www.pubmed.gov), where 15299515 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15299515}}
==About this Structure==
==About this Structure==
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[[Category: Wierenga, R K.]]
[[Category: Wierenga, R K.]]
[[Category: Intramolecular oxidoreductase]]
[[Category: Intramolecular oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:17:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:25:42 2008''

Revision as of 12:25, 27 July 2008

Image:1tre.png

Template:STRUCTURE 1tre

THE STRUCTURE OF TRIOSEPHOSPHATE ISOMERASE FROM ESCHERICHIA COLI DETERMINED AT 2.6 ANGSTROM RESOLUTION

Template:ABSTRACT PUBMED 15299515

About this Structure

1TRE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of triosephosphate isomerase from Escherichia coli determined at 2.6 A resolution., Noble ME, Zeelen JP, Wierenga RK, Mainfroid V, Goraj K, Gohimont AC, Martial JA, Acta Crystallogr D Biol Crystallogr. 1993 Jul 1;49(Pt 4):403-17. PMID:15299515

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