1tue

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{{STRUCTURE_1tue| PDB=1tue | SCENE= }}
{{STRUCTURE_1tue| PDB=1tue | SCENE= }}
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'''The X-ray Structure of the Papillomavirus Helicase in Complex with its Molecular Matchmaker E2'''
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===The X-ray Structure of the Papillomavirus Helicase in Complex with its Molecular Matchmaker E2===
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==Overview==
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DNA replication of the papillomaviruses is specified by cooperative binding of two proteins to the ori site: the enhancer E2 and the viral initiator E1, a distant member of the AAA+ family of proteins. Formation of this prereplication complex is an essential step toward the construction of a functional, multimeric E1 helicase and DNA melting. To understand how E2 interacts with E1 to regulate this process, we have solved the X-ray structure of a complex containing the HPV18 E2 activation domain bound to the helicase domain of E1. Modeling the monomers of E1 to a hexameric helicase shows that E2 blocks hexamerization of E1 by shielding a region of the E1 oligomerization surface and stabilizing a conformation of E1 that is incompatible with ATP binding. Further biochemical experiments and structural analysis show that ATP is an allosteric effector of the dissociation of E2 from E1. Our data provide the first molecular insights into how a protein can regulate the assembly of an oligomeric AAA+ complex and explain at a structural level why E2, after playing a matchmaker role by guiding E1 to the DNA, must dissociate for subsequent steps of initiation to occur. Building on previously proposed ideas, we discuss how our data advance current models for the conversion of E1 in the prereplication complex to a hexameric helicase assembly.
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(as it appears on PubMed at http://www.pubmed.gov), where 15289463 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15289463}}
==About this Structure==
==About this Structure==
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[[Category: Human papillomavirus]]
[[Category: Human papillomavirus]]
[[Category: Replication]]
[[Category: Replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:22:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:17:56 2008''

Revision as of 00:17, 28 July 2008

Image:1tue.png

Template:STRUCTURE 1tue

The X-ray Structure of the Papillomavirus Helicase in Complex with its Molecular Matchmaker E2

Template:ABSTRACT PUBMED 15289463

About this Structure

1TUE is a Protein complex structure of sequences from Human papillomavirus type 18. Full crystallographic information is available from OCA.

Reference

The X-ray structure of the papillomavirus helicase in complex with its molecular matchmaker E2., Abbate EA, Berger JM, Botchan MR, Genes Dev. 2004 Aug 15;18(16):1981-96. Epub 2004 Aug 2. PMID:15289463

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