1xi2
From Proteopedia
(New page: 200px<br /> <applet load="1xi2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xi2, resolution 1.5Å" /> '''Quinone Reductase 2 ...) |
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| - | [[Image:1xi2. | + | [[Image:1xi2.jpg|left|200px]]<br /><applet load="1xi2" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1xi2" size=" | + | |
caption="1xi2, resolution 1.5Å" /> | caption="1xi2, resolution 1.5Å" /> | ||
'''Quinone Reductase 2 in Complex with Cancer Prodrug CB1954'''<br /> | '''Quinone Reductase 2 in Complex with Cancer Prodrug CB1954'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XI2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, FAD and CB1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1XI2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=CB1:'>CB1</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XI2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: qr2]] | [[Category: qr2]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:09:20 2008'' |
Revision as of 15:09, 15 February 2008
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Quinone Reductase 2 in Complex with Cancer Prodrug CB1954
Overview
CB1954 is a cancer pro-drug that can be activated through reduction by, Escherichia coli nitro-reductases and quinone reductases. Human quinone, reductase 2 is very efficient in the activation of CB1954, approximately, 3000 times more efficient than human QR1 in terms of k(cat)/K(m). We have, solved the three-dimensional structure of QR2 in complex with CB1954 to a, nominal resolution of 1.5A. The complex structure indicates the, essentiality of the two nitro groups: one nitro group forms hydrogen bonds, with the side-chain of Asn161 of QR2 to hold the other nitro group in, position for the reduction. We further conclude that residue 161, an Asn, in QR2 and a His in QR1, is critical in differentiating the substrate, specificities of these two enzymes. Mutation of Asn161 to His161 in QR2, resulted in the total loss of the enzymatic activity towards activation of, CB1954, whereas the rates of reduction towards menadione are not altered.
About this Structure
1XI2 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of quinone reductase 2 in complex with cancer prodrug CB1954., Fu Y, Buryanovskyy L, Zhang Z, Biochem Biophys Res Commun. 2005 Oct 14;336(1):332-8. PMID:16129418
Page seeded by OCA on Fri Feb 15 17:09:20 2008
Categories: Homo sapiens | Single protein | Buryanovskyy, L. | Fu, Y. | Zhang, Z. | CB1 | FAD | ZN | Cb1954 | Qr2
