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1u04

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[[Image:1u04.gif|left|200px]]
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[[Image:1u04.png|left|200px]]
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==Overview==
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{{ABSTRACT_15284453}}
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Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.
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==About this Structure==
==About this Structure==
1U04 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U04 OCA].
1U04 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U04 OCA].
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==Reference==
 
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Crystal structure of Argonaute and its implications for RISC slicer activity., Song JJ, Smith SK, Hannon GJ, Joshua-Tor L, Science. 2004 Sep 3;305(5689):1434-7. Epub 2004 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15284453 15284453]
 
[[Category: Pyrococcus furiosus dsm 3638]]
[[Category: Pyrococcus furiosus dsm 3638]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Silencing]]
[[Category: Silencing]]
[[Category: Slicer]]
[[Category: Slicer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:34:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:02:39 2008''

Revision as of 15:02, 26 June 2008

Image:1u04.png

Template:STRUCTURE 1u04

Crystal structure of full length Argonaute from Pyrococcus furiosus


Template:ABSTRACT 15284453

About this Structure

1U04 is a Single protein structure of sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:02:39 2008

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