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1u0c

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[[Image:1u0c.gif|left|200px]]
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{{Seed}}
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[[Image:1u0c.png|left|200px]]
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{{STRUCTURE_1u0c| PDB=1u0c | SCENE= }}
{{STRUCTURE_1u0c| PDB=1u0c | SCENE= }}
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'''Y33C Mutatant of Homing endonuclease I-CreI'''
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===Y33C Mutatant of Homing endonuclease I-CreI===
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==Overview==
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Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting. These studies require that naturally occurring enzymes be redesigned to recognize novel target sites. Here, we report studies in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains corresponding to contact points to distinct base-pairs in its target site. The resulting enzyme constructs drive specific elimination of selected DNA targets in vivo and display shifted specificities of DNA binding and cleavage in vitro. Crystal structures of two of these constructs demonstrate that substitution of individual side-chain/DNA contact patterns can occur with almost no structural deformation or rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and specificity.
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The line below this paragraph, {{ABSTRACT_PUBMED_15313605}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15313605 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15313605}}
==About this Structure==
==About this Structure==
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[[Category: Dna endonuclease i-crei]]
[[Category: Dna endonuclease i-crei]]
[[Category: Protein/dna]]
[[Category: Protein/dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:35:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 6 12:03:25 2008''

Revision as of 09:03, 6 July 2008

Image:1u0c.png

Template:STRUCTURE 1u0c

Y33C Mutatant of Homing endonuclease I-CreI

Template:ABSTRACT PUBMED 15313605

About this Structure

1U0C is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

Reference

Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:15313605

Page seeded by OCA on Sun Jul 6 12:03:25 2008

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