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1u10

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[[Image:1u10.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1u10| PDB=1u10 | SCENE= }}
{{STRUCTURE_1u10| PDB=1u10 | SCENE= }}
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'''MEPA, active form with ZN in P1'''
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===MEPA, active form with ZN in P1===
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==Overview==
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LAS enzymes are a group of metallopeptidases that share an active site architecture and a core folding motif and have been named according to the group members lysostaphin, D-Ala-D-Ala carboxypeptidase and sonic hedgehog. Escherichia coli MepA is a periplasmic, penicillin-insensitive murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond in E. coli peptidoglycan. The enzyme lacks sequence similarity with other peptidases, and is currently classified as a peptidase of unknown fold and catalytic class in all major data bases. Here, we build on our observation that two motifs, characteristic of the newly described LAS group of metallopeptidases, are conserved in MepA-type sequences. We demonstrate that recombinant E. coli MepA is sensitive to metal chelators and that mutations in the predicted Zn2+ ligands His-113, Asp-120, and His-211 inactivate the enzyme. Moreover, we present the crystal structure of MepA. The active site of the enzyme is most similar to the active sites of lysostaphin and D-Ala-D-Ala carboxypeptidase, and the fold is most closely related to the N-domain of sonic hedgehog. We conclude that MepA-type peptidases are LAS enzymes.
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The line below this paragraph, {{ABSTRACT_PUBMED_15292190}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15292190 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15292190}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Peptidoglycan amidase MepA is a LAS metallopeptidase., Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M, J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15292190 15292190]
Peptidoglycan amidase MepA is a LAS metallopeptidase., Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M, J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15292190 15292190]
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Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases., Bochtler M, Odintsov SG, Marcyjaniak M, Sabala I, Protein Sci. 2004 Apr;13(4):854-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15044722 15044722]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Metallopeptidase]]
[[Category: Metallopeptidase]]
[[Category: Peptidoglycan hydrolase]]
[[Category: Peptidoglycan hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:37:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:44:18 2008''

Revision as of 03:44, 29 July 2008

Image:1u10.png

Template:STRUCTURE 1u10

MEPA, active form with ZN in P1

Template:ABSTRACT PUBMED 15292190

About this Structure

1U10 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Peptidoglycan amidase MepA is a LAS metallopeptidase., Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M, J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. PMID:15292190

Similar active sites in lysostaphins and D-Ala-D-Ala metallopeptidases., Bochtler M, Odintsov SG, Marcyjaniak M, Sabala I, Protein Sci. 2004 Apr;13(4):854-61. PMID:15044722

Page seeded by OCA on Tue Jul 29 06:44:18 2008

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