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1u4n

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{{STRUCTURE_1u4n| PDB=1u4n | SCENE= }}
{{STRUCTURE_1u4n| PDB=1u4n | SCENE= }}
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'''Crystal Structure Analysis of the M211S/R215L EST2 mutant'''
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===Crystal Structure Analysis of the M211S/R215L EST2 mutant===
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==Overview==
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Esterase 2 (EST2) from the thermophilic eubacterium Alicyclobacillus acidocaldarius is a thermostable serine hydrolase belonging to the H group of the esterase/lipase family. This enzyme hydrolyzes monoacylesters of different acyl-chain length and various compounds with industrial interest. EST2 displays an optimal temperature at 70 degrees C and maximal activity with pNP-esters having acyl-chain bearing from six to eight carbon atoms. EST2 mutants with different substrate specificity were also designed, generated by site-directed mutagenesis, and biochemically characterized. To better define at structural level the enzyme reaction mechanism, a crystallographic analysis of one of these mutants, namely M211S/R215L, was undertaken. Here we report its three-dimensional structure at 2.10A resolution. Structural analysis of the enzyme revealed an unexpected dimer formation as a consequence of a domain-swapping event involving its N-terminal region. This phenomenon was absent in the case of the enzyme bound to an irreversible inhibitor having optimal substrate structural features. A detailed comparison of the enzyme structures before and following binding to this molecule showed a movement of the N-terminal helices resulting from a trans-cis isomerization of the F37-P38 peptide bond. These findings suggest that this carboxylesterase presents two distinct structural arrangements reminiscent of the open and closed forms already reported for lipases. Potential biological implications associated with the observed quaternary reorganization are here discussed in light of the biochemical properties of other lipolytic members of the H group.
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{{ABSTRACT_PUBMED_15381425}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family., De Simone G, Menchise V, Alterio V, Mandrich L, Rossi M, Manco G, Pedone C, J Mol Biol. 2004 Oct 8;343(1):137-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15381425 15381425]
The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family., De Simone G, Menchise V, Alterio V, Mandrich L, Rossi M, Manco G, Pedone C, J Mol Biol. 2004 Oct 8;343(1):137-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15381425 15381425]
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A snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase., De Simone G, Galdiero S, Manco G, Lang D, Rossi M, Pedone C, J Mol Biol. 2000 Nov 10;303(5):761-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11061974 11061974]
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A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis., De Simone G, Mandrich L, Menchise V, Giordano V, Febbraio F, Rossi M, Pedone C, Manco G, J Biol Chem. 2004 Feb 20;279(8):6815-23. Epub 2003 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14617621 14617621]
[[Category: Alicyclobacillus acidocaldarius]]
[[Category: Alicyclobacillus acidocaldarius]]
[[Category: Carboxylesterase]]
[[Category: Carboxylesterase]]
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[[Category: Alpha/beta hydrolase fold]]
[[Category: Alpha/beta hydrolase fold]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:45:29 2008''

Revision as of 13:45, 27 July 2008

Image:1u4n.png

Template:STRUCTURE 1u4n

Crystal Structure Analysis of the M211S/R215L EST2 mutant

Template:ABSTRACT PUBMED 15381425

About this Structure

1U4N is a Single protein structure of sequence from Alicyclobacillus acidocaldarius. Full crystallographic information is available from OCA.

Reference

The crystal structure of an EST2 mutant unveils structural insights on the H group of the carboxylesterase/lipase family., De Simone G, Menchise V, Alterio V, Mandrich L, Rossi M, Manco G, Pedone C, J Mol Biol. 2004 Oct 8;343(1):137-46. PMID:15381425

A snapshot of a transition state analogue of a novel thermophilic esterase belonging to the subfamily of mammalian hormone-sensitive lipase., De Simone G, Galdiero S, Manco G, Lang D, Rossi M, Pedone C, J Mol Biol. 2000 Nov 10;303(5):761-71. PMID:11061974

A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis., De Simone G, Mandrich L, Menchise V, Giordano V, Febbraio F, Rossi M, Pedone C, Manco G, J Biol Chem. 2004 Feb 20;279(8):6815-23. Epub 2003 Nov 15. PMID:14617621

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