1u78

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{{STRUCTURE_1u78| PDB=1u78 | SCENE= }}
{{STRUCTURE_1u78| PDB=1u78 | SCENE= }}
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'''Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA'''
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===Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA===
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==Overview==
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The bipartite DNA-binding domain of Tc3 transposase, Tc3A, was crystallized in complex with its transposon recognition sequence. In the structure the two DNA-binding domains form structurally related helix-turn-helix (HTH) motifs. They both bind to the major groove on a single DNA oligomer, separated by a linker that interacts closely with the minor groove. The structure resembles that of the transcription factor Pax6 DNA-binding domain, but the relative orientation of the HTH-domain is different. The DNA conformation is distorted, characterized by local narrowing of the minor groove and bends at both ends. The protein-DNA recognition takes place through base and backbone contacts, as well as shape-recognition of the distortions in the DNA. Charged interactions are primarily found in the N-terminal domain and the linker indicating that these may form the initial contact area. Two independent dimer interfaces could be relevant for bringing together transposon ends and for binding to a direct repeat site in the transposon end. In contrast to the Tn5 synaptic complex, the two Tc3A DNA-binding domains bind to a single Tc3 transposon end.
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(as it appears on PubMed at http://www.pubmed.gov), where 15304566 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15304566}}
==About this Structure==
==About this Structure==
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[[Category: Transposase]]
[[Category: Transposase]]
[[Category: Transposon dna]]
[[Category: Transposon dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:08:58 2008''

Revision as of 12:08, 27 July 2008

Image:1u78.png

Template:STRUCTURE 1u78

Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA

Template:ABSTRACT PUBMED 15304566

About this Structure

1U78 is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

Reference

Structural analysis of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA., Watkins S, van Pouderoyen G, Sixma TK, Nucleic Acids Res. 2004 Aug 10;32(14):4306-12. Print 2004. PMID:15304566

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