1xu1
From Proteopedia
(New page: 200px<br /> <applet load="1xu1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xu1, resolution 1.90Å" /> '''The crystal structu...) |
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| - | [[Image:1xu1.gif|left|200px]]<br /> | + | [[Image:1xu1.gif|left|200px]]<br /><applet load="1xu1" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1xu1" size=" | + | |
caption="1xu1, resolution 1.90Å" /> | caption="1xu1, resolution 1.90Å" /> | ||
'''The crystal structure of APRIL bound to TACI'''<br /> | '''The crystal structure of APRIL bound to TACI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | TACI is a member of the tumor necrosis factor receptor superfamily and | + | TACI is a member of the tumor necrosis factor receptor superfamily and serves as a key regulator of B cell function. TACI binds two ligands, APRIL and BAFF, with high affinity and contains two cysteine-rich domains (CRDs) in its extracellular region; in contrast, BCMA and BR3, the other known high affinity receptors for APRIL and BAFF, respectively, contain only a single or partial CRD. However, another form of TACI exists wherein the N-terminal CRD is removed by alternative splicing. We find that this shorter form is capable of ligand-induced cell signaling and that the second CRD alone (TACI_d2) contains full affinity for both ligands. Furthermore, we report the solution structure and alanine-scanning mutagenesis of TACI_d2 along with co-crystal structures of APRIL.TACI_d2 and APRIL.BCMA complexes that together reveal the mechanism by which TACI engages high affinity ligand binding through a single CRD, and we highlight sources of ligand-receptor specificity within the APRIL/BAFF system. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XU1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1XU1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XU1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Gordon, N | + | [[Category: Gordon, N C.]] |
| - | [[Category: Hymowitz, S | + | [[Category: Hymowitz, S G.]] |
| - | [[Category: Kelley, R | + | [[Category: Kelley, R F.]] |
[[Category: Pan, B.]] | [[Category: Pan, B.]] | ||
| - | [[Category: Patel, D | + | [[Category: Patel, D R.]] |
[[Category: Runyon, S.]] | [[Category: Runyon, S.]] | ||
| - | [[Category: Shriver, S | + | [[Category: Shriver, S K.]] |
| - | [[Category: Skelton, N | + | [[Category: Skelton, N J.]] |
| - | [[Category: Starovasnik, M | + | [[Category: Starovasnik, M A.]] |
| - | [[Category: Wallweber, H | + | [[Category: Wallweber, H J.A.]] |
[[Category: Yan, M.]] | [[Category: Yan, M.]] | ||
[[Category: Yin, J.]] | [[Category: Yin, J.]] | ||
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[[Category: tnfsf]] | [[Category: tnfsf]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:39 2008'' |
Revision as of 13:58, 21 February 2008
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The crystal structure of APRIL bound to TACI
Contents |
Overview
TACI is a member of the tumor necrosis factor receptor superfamily and serves as a key regulator of B cell function. TACI binds two ligands, APRIL and BAFF, with high affinity and contains two cysteine-rich domains (CRDs) in its extracellular region; in contrast, BCMA and BR3, the other known high affinity receptors for APRIL and BAFF, respectively, contain only a single or partial CRD. However, another form of TACI exists wherein the N-terminal CRD is removed by alternative splicing. We find that this shorter form is capable of ligand-induced cell signaling and that the second CRD alone (TACI_d2) contains full affinity for both ligands. Furthermore, we report the solution structure and alanine-scanning mutagenesis of TACI_d2 along with co-crystal structures of APRIL.TACI_d2 and APRIL.BCMA complexes that together reveal the mechanism by which TACI engages high affinity ligand binding through a single CRD, and we highlight sources of ligand-receptor specificity within the APRIL/BAFF system.
Disease
Known diseases associated with this structure: Common variable immunodeficiency OMIM:[604907], Immunoglobulin A deficiency OMIM:[604907]
About this Structure
1XU1 is a Protein complex structure of sequences from Homo sapiens and Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Structures of APRIL-receptor complexes: like BCMA, TACI employs only a single cysteine-rich domain for high affinity ligand binding., Hymowitz SG, Patel DR, Wallweber HJ, Runyon S, Yan M, Yin J, Shriver SK, Gordon NC, Pan B, Skelton NJ, Kelley RF, Starovasnik MA, J Biol Chem. 2005 Feb 25;280(8):7218-27. Epub 2004 Nov 12. PMID:15542592
Page seeded by OCA on Thu Feb 21 15:58:39 2008
Categories: Homo sapiens | Mus musculus | Protein complex | Gordon, N C. | Hymowitz, S G. | Kelley, R F. | Pan, B. | Patel, D R. | Runyon, S. | Shriver, S K. | Skelton, N J. | Starovasnik, M A. | Wallweber, H J.A. | Yan, M. | Yin, J. | NI | Crd | Cysteine-rich | Cytokine | Jelly-roll | Receptor | Tnfsf
