1uaw

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{{STRUCTURE_1uaw| PDB=1uaw | SCENE= }}
{{STRUCTURE_1uaw| PDB=1uaw | SCENE= }}
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'''Solution structure of the N-terminal RNA-binding domain of mouse Musashi1'''
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===Solution structure of the N-terminal RNA-binding domain of mouse Musashi1===
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==Overview==
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Musashi1 is an RNA-binding protein abundantly expressed in the developing mouse central nervous system. Its restricted expression in neural precursor cells suggests that it is involved in maintenance of the character of progenitor cells. Musashi1 contains two ribonucleoprotein-type RNA-binding domains (RBDs), RBD1 and RBD2, the affinity to RNA of RBD1 being much higher than that of RBD2. We previously reported the structure and mode of interaction with RNA of RBD2. Here, we have determined the structure and mode of interaction with RNA of RBD1. We have also analyzed the surface electrostatic potential and backbone dynamics of both RBDs. The two RBDs exhibit the same ribo-nucleoprotein-type fold and commonly make contact with RNA on the beta-sheet side. On the other hand, there is a remarkable difference in surface electrostatic potential, the beta-sheet of RBD1 being positively charged, which is favorable for binding negatively charged RNA, but that of RBD2 being almost neutral. There is also a difference in backbone dynamics, the central portion of the beta-sheet of RBD1 being flexible, but that of RBD2 not being flexible. The flexibility of RBD1 may be utilized in the recognition process to facilitate an induced fit. Thus, comparative studies have revealed the origin of the higher affinity of RBD1 than that of RBD2 and indicated that the affinity of an RBD to RNA is not governed by its fold alone but is also determined by its surface electrostatic potential and/or backbone dynamics. The biological role of RBD2 with lower affinity is also discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 12907678 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12907678}}
==About this Structure==
==About this Structure==
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1UAW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAW OCA].
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1UAW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAW OCA].
==Reference==
==Reference==
Origin of higher affinity to RNA of the N-terminal RNA-binding domain than that of the C-terminal one of a mouse neural protein, musashi1, as revealed by comparison of their structures, modes of interaction, surface electrostatic potentials, and backbone dynamics., Miyanoiri Y, Kobayashi H, Imai T, Watanabe M, Nagata T, Uesugi S, Okano H, Katahira M, J Biol Chem. 2003 Oct 17;278(42):41309-15. Epub 2003 Aug 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12907678 12907678]
Origin of higher affinity to RNA of the N-terminal RNA-binding domain than that of the C-terminal one of a mouse neural protein, musashi1, as revealed by comparison of their structures, modes of interaction, surface electrostatic potentials, and backbone dynamics., Miyanoiri Y, Kobayashi H, Imai T, Watanabe M, Nagata T, Uesugi S, Okano H, Katahira M, J Biol Chem. 2003 Oct 17;278(42):41309-15. Epub 2003 Aug 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12907678 12907678]
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Structure, backbone dynamics and interactions with RNA of the C-terminal RNA-binding domain of a mouse neural RNA-binding protein, Musashi1., Nagata T, Kanno R, Kurihara Y, Uesugi S, Imai T, Sakakibara S, Okano H, Katahira M, J Mol Biol. 1999 Mar 26;287(2):315-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10080895 10080895]
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Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1., Kurihara Y, Nagata T, Imai T, Hiwatashi A, Horiuchi M, Sakakibara S, Katahira M, Okano H, Uesugi S, Gene. 1997 Feb 20;186(1):21-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9047340 9047340]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Watanabe, M.]]
[[Category: Watanabe, M.]]
[[Category: Rnp-type structure]]
[[Category: Rnp-type structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:58:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:06:39 2008''

Revision as of 05:06, 28 July 2008

Image:1uaw.png

Template:STRUCTURE 1uaw

Solution structure of the N-terminal RNA-binding domain of mouse Musashi1

Template:ABSTRACT PUBMED 12907678

About this Structure

1UAW is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Origin of higher affinity to RNA of the N-terminal RNA-binding domain than that of the C-terminal one of a mouse neural protein, musashi1, as revealed by comparison of their structures, modes of interaction, surface electrostatic potentials, and backbone dynamics., Miyanoiri Y, Kobayashi H, Imai T, Watanabe M, Nagata T, Uesugi S, Okano H, Katahira M, J Biol Chem. 2003 Oct 17;278(42):41309-15. Epub 2003 Aug 7. PMID:12907678

Structure, backbone dynamics and interactions with RNA of the C-terminal RNA-binding domain of a mouse neural RNA-binding protein, Musashi1., Nagata T, Kanno R, Kurihara Y, Uesugi S, Imai T, Sakakibara S, Okano H, Katahira M, J Mol Biol. 1999 Mar 26;287(2):315-30. PMID:10080895

Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1., Kurihara Y, Nagata T, Imai T, Hiwatashi A, Horiuchi M, Sakakibara S, Katahira M, Okano H, Uesugi S, Gene. 1997 Feb 20;186(1):21-7. PMID:9047340

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