1ue8

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{{STRUCTURE_1ue8| PDB=1ue8 | SCENE= }}
{{STRUCTURE_1ue8| PDB=1ue8 | SCENE= }}
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'''Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii'''
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===Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii===
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==Overview==
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Cytochrome P450 from thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (P450st) has been expressed in Escherichia coli and purified at high homogeneity. P450st was crystallized in an orthorhombic system with the space group P2(1)2(1)2(1) and cell dimensions of a=53.6 A, b=55.1 A, and c=130.9 A, and the structure was determined at a 3.0 A resolution. The final R-factor was 0.194 (Rfree=0.235). Structural comparison with cytochrome P450 from S. solfataricus (CYP119) suggests that the region composed of the F to G helices and the Cl- binding site is responsible for the affinity for a ligand coordinating heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide (DDAB) film on a plastic formed carbon (PFC) electrode has also been demonstrated. A quasi-reversible redox response has been observed even at elevated temperatures of up to 80 degrees C.
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{{ABSTRACT_PUBMED_15219985}}
==About this Structure==
==About this Structure==
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[[Category: Alpha-domain]]
[[Category: Alpha-domain]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:06:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:49:13 2008''

Revision as of 15:49, 28 July 2008

Image:1ue8.png

Template:STRUCTURE 1ue8

Crystal Structure of Thermophilic Cytochrome P450 from Sulfolobus tokodaii

Template:ABSTRACT PUBMED 15219985

About this Structure

1UE8 is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.

Reference

Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7., Oku Y, Ohtaki A, Kamitori S, Nakamura N, Yohda M, Ohno H, Kawarabayasi Y, J Inorg Biochem. 2004 Jul;98(7):1194-9. PMID:15219985

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