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1uec

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{{STRUCTURE_1uec| PDB=1uec | SCENE= }}
{{STRUCTURE_1uec| PDB=1uec | SCENE= }}
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'''Crystal structure of autoinhibited form of tandem SH3 domain of p47phox'''
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===Crystal structure of autoinhibited form of tandem SH3 domain of p47phox===
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==Overview==
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The phagocyte NADPH oxidase is a multisubunit enzyme responsible for the production of reactive oxygen species. p47(phox) is a cytosolic component of the NADPH oxidase and plays an important role in the assembly of the activated complex. The structural determination of the tandem SH3 domains of p47(phox) is crucial for elucidation of the molecular mechanism of the activation of p47(phox). We determined the X-ray crystal structure of the tandem SH3 domains with the polybasic/autoinhibitory region (PBR/AIR) of p47(phox). The GAPPR sequence involved in PBR/AIR forms a left-handed polyproline type-II helix (PPII) and interacts with the conserved SH3 binding surfaces of the SH3 domains simultaneously. These SH3 domains are related by a 2-fold pseudosymmetry axis at the centre of the binding groove and interact with the single PPII helix formed by the GAPPR sequence with opposite orientation. In addition, a number of intra-molecular interactions among the SH3 domains, PBR/AIR and the linker tightly hold the architecture of the tandem SH3 domains into the compact structure and stabilize the autoinhibited form synergistically. Phosphorylation of the serine residues in PBR/AIR could destabilize and successively release the intra-molecular interactions. Thus, the overall structure could be rearranged from the autoinhibitory conformation to the active conformation and the PPII ligand binding surfaces on the SH3 domains are now unmasked, which enables their interaction with the target sequence in p22(phox).
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(as it appears on PubMed at http://www.pubmed.gov), where 15147273 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15147273}}
==About this Structure==
==About this Structure==
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[[Category: Phagocyte]]
[[Category: Phagocyte]]
[[Category: Sh3 domain]]
[[Category: Sh3 domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:06:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:34:59 2008''

Revision as of 17:35, 27 July 2008

Image:1uec.png

Template:STRUCTURE 1uec

Crystal structure of autoinhibited form of tandem SH3 domain of p47phox

Template:ABSTRACT PUBMED 15147273

About this Structure

1UEC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase., Yuzawa S, Suzuki NN, Fujioka Y, Ogura K, Sumimoto H, Inagaki F, Genes Cells. 2004 May;9(5):443-56. PMID:15147273

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