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1y02
From Proteopedia
(New page: 200px<br /> <applet load="1y02" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y02, resolution 1.8Å" /> '''Crystal Structure of...) |
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| - | [[Image:1y02.gif|left|200px]]<br /> | + | [[Image:1y02.gif|left|200px]]<br /><applet load="1y02" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1y02" size=" | + | |
caption="1y02, resolution 1.8Å" /> | caption="1y02, resolution 1.8Å" /> | ||
'''Crystal Structure of a FYVE-type domain from caspase regulator CARP2'''<br /> | '''Crystal Structure of a FYVE-type domain from caspase regulator CARP2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The caspase-associated ring proteins (CARP1 and CARP2) are distinguished | + | The caspase-associated ring proteins (CARP1 and CARP2) are distinguished from other caspase regulators by the presence of a FYVE-type zinc finger domain. FYVE-type domains are divided into two known classes: FYVE domains that specifically bind to phosphatidylinositol 3-phosphate in lipid bilayers and FYVE-related domains of undetermined function. Here, we report the crystal structure of the N-terminal region of CARP2 (44-139) including the FYVE-type domain and its associated helical bundle at 1.7 A resolution. The structure reveals a cramped phosphoinositide binding pocket and a blunted membrane insertion loop. These structural features indicate that the domain is not optimized to bind to phosphoinositides or insert into lipid bilayers. The CARP2 FYVE-like domain thus defines a third subfamily of FYVE-type domains that are functionally and structurally distinct. Structural analyses provide insights into the possible function of this unique subfamily of FYVE-type domains. |
==About this Structure== | ==About this Structure== | ||
| - | 1Y02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1Y02 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y02 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gu, L.]] | [[Category: Gu, L.]] | ||
[[Category: Shi, Y.]] | [[Category: Shi, Y.]] | ||
| - | [[Category: Shiozaki, E | + | [[Category: Shiozaki, E N.]] |
| - | [[Category: Tibbetts, M | + | [[Category: Tibbetts, M D.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: carp2]] | [[Category: carp2]] | ||
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[[Category: zinc-binding module]] | [[Category: zinc-binding module]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:00:27 2008'' |
Revision as of 14:00, 21 February 2008
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Crystal Structure of a FYVE-type domain from caspase regulator CARP2
Overview
The caspase-associated ring proteins (CARP1 and CARP2) are distinguished from other caspase regulators by the presence of a FYVE-type zinc finger domain. FYVE-type domains are divided into two known classes: FYVE domains that specifically bind to phosphatidylinositol 3-phosphate in lipid bilayers and FYVE-related domains of undetermined function. Here, we report the crystal structure of the N-terminal region of CARP2 (44-139) including the FYVE-type domain and its associated helical bundle at 1.7 A resolution. The structure reveals a cramped phosphoinositide binding pocket and a blunted membrane insertion loop. These structural features indicate that the domain is not optimized to bind to phosphoinositides or insert into lipid bilayers. The CARP2 FYVE-like domain thus defines a third subfamily of FYVE-type domains that are functionally and structurally distinct. Structural analyses provide insights into the possible function of this unique subfamily of FYVE-type domains.
About this Structure
1Y02 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2., Tibbetts MD, Shiozaki EN, Gu L, McDonald ER 3rd, El-Deiry WS, Shi Y, Structure. 2004 Dec;12(12):2257-63. PMID:15576038
Page seeded by OCA on Thu Feb 21 16:00:27 2008
