1uk9

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{{STRUCTURE_1uk9| PDB=1uk9 | SCENE= }}
{{STRUCTURE_1uk9| PDB=1uk9 | SCENE= }}
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'''Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with isovalerate'''
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===Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with isovalerate===
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==Overview==
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Meta-cleavage product hydrolase (MCP-hydrolase) is one of the key enzymes in the microbial degradation of aromatic compounds. MCP-hydrolase produces 2-hydroxypenta-2,4-dienoate and various organic acids, according to the C6 substituent of the substrate. Comprehensive analysis of the substrate specificity of the MCP-hydrolase from Pseudomonas fluorescens IP01 (CumD) was carried out by determining the kinetic parameters for nine substrates and crystal structures complexed with eight cleavage products. CumD preferred substrates with long non-branched C6 substituents, but did not effectively hydrolyze a substrate with a phenyl group. Superimposition of the complex structures indicated that benzoate was bound in a significantly different direction than other aliphatic cleavage products. The directions of the bound organic acids appeared to be related with the k(cat) values of the corresponding substrates. The Ile139 and Trp143 residues on helix alpha4 appeared to cause steric hindrance with the aromatic ring of the substrate, which hampers base-catalyzed attack by water.
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{{ABSTRACT_PUBMED_15784976}}
==About this Structure==
==About this Structure==
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[[Category: Pseudomonas fluorescens ip01]]
[[Category: Pseudomonas fluorescens ip01]]
[[Category: Substrate specificity]]
[[Category: Substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:20:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:06:33 2008''

Revision as of 09:06, 28 July 2008

Image:1uk9.png

Template:STRUCTURE 1uk9

Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with isovalerate

Template:ABSTRACT PUBMED 15784976

About this Structure

1UK9 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products., Fushinobu S, Jun SY, Hidaka M, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T, Biosci Biotechnol Biochem. 2005 Mar;69(3):491-8. PMID:15784976

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