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1um2

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{{STRUCTURE_1um2| PDB=1um2 | SCENE= }}
{{STRUCTURE_1um2| PDB=1um2 | SCENE= }}
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'''Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment'''
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===Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment===
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==Overview==
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Protein splicing precisely excises out an internal intein segment from a protein precursor, and concomitantly ligates the N- and C-terminal extein polypeptides flanking the intein. A recombinant X10SNS bearing N- and C-extein polypeptides has been prepared for the intein endonuclease derived from the Saccharomyces cerevisiae VMA1 gene. X10SNS has replacements of C284S, H362N and C738S, and forms the intein and extein segments in the crystal lattice. The crystal structure of X10SNS revealed a linkage between the N- and C-extein segments, and showed that the C284 amino group of the resultant intein segment is in interaction with the G283 O atom of the N-extein segment. A mechanism for the final S --&gt; N acyl shift step proposes that a tetrahedral intermediate involves a five-membered thiazolidine ring at G283-C738 junction. An oxyanion of the thiazolidine intermediate is to be stabilized by the C284 N atom.
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The line below this paragraph, {{ABSTRACT_PUBMED_14646148}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_14646148}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646148 14646148]
Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14646148 14646148]
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Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides., Mizutani R, Nogami S, Kawasaki M, Ohya Y, Anraku Y, Satow Y, J Mol Biol. 2002 Mar 1;316(4):919-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884132 11884132]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Vde]]
[[Category: Vde]]
[[Category: Vma1-derived endonuclease]]
[[Category: Vma1-derived endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:24:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:01:57 2008''

Revision as of 03:01, 28 July 2008

Image:1um2.png

Template:STRUCTURE 1um2

Crystal Structure of the Vma1-Derived Endonuclease with the Ligated Extein Segment

Template:ABSTRACT PUBMED 14646148

About this Structure

1UM2 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Protein splicing of yeast VMA1-derived endonuclease via thiazolidine intermediates., Mizutani R, Anraku Y, Satow Y, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):109-12. Epub 2003 Nov 28. PMID:14646148

Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides., Mizutani R, Nogami S, Kawasaki M, Ohya Y, Anraku Y, Satow Y, J Mol Biol. 2002 Mar 1;316(4):919-29. PMID:11884132

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