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1uo9

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{{STRUCTURE_1uo9| PDB=1uo9 | SCENE= }}
{{STRUCTURE_1uo9| PDB=1uo9 | SCENE= }}
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'''DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE'''
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===DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE===
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==Overview==
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Deacetoxycephalosporin-C synthase (DAOCS) is a mononuclear ferrous enzyme that transforms penicillins into cephalosporins by inserting a carbon atom into the penicillin nucleus. In the first half-reaction, dioxygen and 2-oxoglutarate produce a reactive iron-oxygen species, succinate and CO2. The oxidizing iron species subsequently reacts with penicillin to give cephalosporin and water. Here we describe high-resolution structures for ferrous DAOCS in complex with penicillins, the cephalosporin product, the cosubstrate and the coproduct. Steady-state kinetic data, quantum-chemical calculations and the new structures indicate a reaction sequence in which a 'booby-trapped' oxidizing species is formed. This species is stabilized by the negative charge of succinate on the iron. The binding sites of succinate and penicillin overlap, and when penicillin replaces succinate, it removes the stabilizing charge, eliciting oxidative attack on itself. Requisite groups of penicillin are within 1 A of the expected position of a ferryl oxygen in the enzyme-penicillin complex.
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The line below this paragraph, {{ABSTRACT_PUBMED_14718929}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14718929 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14718929}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Vitamin c]]
[[Category: Vitamin c]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:29:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:03:08 2008''

Revision as of 07:03, 29 July 2008

Image:1uo9.png

Template:STRUCTURE 1uo9

DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE

Template:ABSTRACT PUBMED 14718929

About this Structure

1UO9 is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference

The structural basis of cephalosporin formation in a mononuclear ferrous enzyme., Valegard K, Terwisscha van Scheltinga AC, Dubus A, Ranghino G, Oster LM, Hajdu J, Andersson I, Nat Struct Mol Biol. 2004 Jan;11(1):95-101. Epub 2003 Dec 29. PMID:14718929

Page seeded by OCA on Tue Jul 29 10:03:08 2008

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