1urq

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[[Image:1urq.gif|left|200px]]
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{{STRUCTURE_1urq| PDB=1urq | SCENE= }}
{{STRUCTURE_1urq| PDB=1urq | SCENE= }}
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'''CRYSTAL STRUCTURE OF NEURONAL Q-SNARES IN COMPLEX WITH R-SNARE MOTIF OF TOMOSYN'''
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===CRYSTAL STRUCTURE OF NEURONAL Q-SNARES IN COMPLEX WITH R-SNARE MOTIF OF TOMOSYN===
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==Overview==
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Upon Ca2+ influx synaptic vesicles fuse with the plasma membrane and release their neurotransmitter cargo into the synaptic cleft. Key players during this process are the Q-SNAREs syntaxin 1a and SNAP-25 and the R-SNARE synaptobrevin 2. It is thought that these membrane proteins gradually assemble into a tight trans-SNARE complex between vesicular and plasma membrane, ultimately leading to membrane fusion. Tomosyn is a soluble protein of 130 kDa that contains a COOH-terminal R-SNARE motif but lacks a transmembrane anchor. Its R-SNARE motif forms a stable core SNARE complex with syntaxin 1a and SNAP-25. Here we present the crystal structure of this core tomosyn SNARE complex at 2.0-A resolution. It consists of a four-helical bundle very similar to that of the SNARE complex containing synaptobrevin. Most differences are found on the surface, where they prevented tight binding of complexin. Both complexes form with similar rates as assessed by CD spectroscopy. In addition, synaptobrevin cannot displace the tomosyn helix from the tight complex and vice versa, indicating that both SNARE complexes represent end products. Moreover, data bank searches revealed that the R-SNARE motif of tomosyn is highly conserved throughout all eukaryotic kingdoms. This suggests that the formation of a tight SNARE complex is important for the function of tomosyn.
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(as it appears on PubMed at http://www.pubmed.gov), where 15316007 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15316007}}
==About this Structure==
==About this Structure==
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[[Category: Four helical bundle]]
[[Category: Four helical bundle]]
[[Category: Tomosyn-snare complex]]
[[Category: Tomosyn-snare complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:35:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:24:23 2008''

Revision as of 13:24, 29 July 2008

Image:1urq.png

Template:STRUCTURE 1urq

CRYSTAL STRUCTURE OF NEURONAL Q-SNARES IN COMPLEX WITH R-SNARE MOTIF OF TOMOSYN

Template:ABSTRACT PUBMED 15316007

About this Structure

1URQ is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis for the inhibitory role of tomosyn in exocytosis., Pobbati AV, Razeto A, Boddener M, Becker S, Fasshauer D, J Biol Chem. 2004 Nov 5;279(45):47192-200. Epub 2004 Aug 16. PMID:15316007

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