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| | {{STRUCTURE_1uty| PDB=1uty | SCENE= }} | | {{STRUCTURE_1uty| PDB=1uty | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)'''
| + | ===CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)=== |
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| - | ==Overview==
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| - | Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15155766}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15155766 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15155766}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Rna binding protein]] | | [[Category: Rna binding protein]] |
| | [[Category: Viral protein]] | | [[Category: Viral protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:41:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:39:35 2008'' |
Revision as of 09:39, 28 July 2008
Template:STRUCTURE 1uty
CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)
Template:ABSTRACT PUBMED 15155766
About this Structure
1UTY is a Single protein structure of sequence from Bluetongue virus. Full crystallographic information is available from OCA.
Reference
Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2., Butan C, Van Der Zandt H, Tucker PA, J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:15155766
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