1uvm

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[[Image:1uvm.gif|left|200px]]
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{{Seed}}
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[[Image:1uvm.png|left|200px]]
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{{STRUCTURE_1uvm| PDB=1uvm | SCENE= }}
{{STRUCTURE_1uvm| PDB=1uvm | SCENE= }}
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'''THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 5NT RNA CONFORMATION A'''
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===THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 5NT RNA CONFORMATION A===
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==Overview==
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The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.
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The line below this paragraph, {{ABSTRACT_PUBMED_14962391}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14962391 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14962391}}
==About this Structure==
==About this Structure==
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[[Category: Stuart, D I.]]
[[Category: Stuart, D I.]]
[[Category: Rna-dependent rna polymerase complex with 5nt rna oligonucleotide - conformation some]]
[[Category: Rna-dependent rna polymerase complex with 5nt rna oligonucleotide - conformation some]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:44:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:42:47 2008''

Revision as of 10:42, 28 July 2008

Image:1uvm.png

Template:STRUCTURE 1uvm

THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 5NT RNA CONFORMATION A

Template:ABSTRACT PUBMED 14962391

About this Structure

1UVM is a Protein complex structure. Full crystallographic information is available from OCA.

Reference

The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391

Page seeded by OCA on Mon Jul 28 13:42:47 2008

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