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1uwk

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{{STRUCTURE_1uwk| PDB=1uwk | SCENE= }}
{{STRUCTURE_1uwk| PDB=1uwk | SCENE= }}
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'''THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE'''
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===THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE===
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==Overview==
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Urocanase (EC 4.2.1.49) from Pseudomonas putida was crystallized after removing one of the seven free thiol groups. The crystal structure was solved by multiwavelength anomalous diffraction (MAD) using a seleno-methionine derivative and then refined at 1.14 A resolution. The enzyme is a symmetric homodimer of 2 x 557 amino acid residues with tightly bound NAD+ cofactors. Each subunit consists of a typical NAD-binding domain inserted into a larger core domain that forms the dimer interface. The core domain has a novel chain fold and accommodates the substrate urocanate in a surface depression. The NAD domain sits like a lid on the core domain depression and points with the nicotinamide group to the substrate. Substrate, nicotinamide and five water molecules are completely sequestered in a cavity. Most likely, one of these water molecules hydrates the substrate during catalysis. This cavity has to open for substrate passage, which probably means lifting the NAD domain. The observed atomic arrangement at the active center gives rise to a detailed proposal for the catalytic mechanism that is consistent with published chemical data. As expected, the variability of the residues involved is low, as derived from a family of 58 proteins annotated as urocanases in the data banks. However, one well-embedded member of this family showed a significant deviation at the active center indicating an incorrect annotation.
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(as it appears on PubMed at http://www.pubmed.gov), where 15313616 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15313616}}
==About this Structure==
==About this Structure==
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Urocanase]]
[[Category: Urocanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:46:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:31:16 2008''

Revision as of 06:31, 28 July 2008

Image:1uwk.png

Template:STRUCTURE 1uwk

THE HIGH RESOLUTION STRUCTURE OF UROCANATE HYDRATASE FROM PSEUDOMONAS PUTIDA IN COMPLEX WITH UROCANATE

Template:ABSTRACT PUBMED 15313616

About this Structure

1UWK is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Structure and action of urocanase., Kessler D, Retey J, Schulz GE, J Mol Biol. 2004 Sep 3;342(1):183-94. PMID:15313616

Page seeded by OCA on Mon Jul 28 09:31:16 2008

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